Flow Cytometric Clinical Immunomonitoring Using Peptide–MHC Class II Tetramers: Optimization of Methods and Protocol Development

Flow Cytometric Clinical Immunomonitoring Using Peptide–MHC Class II Tetramers: Optimization of Methods and Protocol Development

With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions, clinical trials of antigen-specific tolerizing immunotherapy have become a reality. Besides safety, it will be essential to gather mechanistic data on responding CD4+ T cells to assess the effects o...

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Bibliographic Details
Main Authors: Diahann T. S. L. Jansen, Nishta Ramnoruth, Khai L. Loh, Jamie Rossjohn, Hugh H. Reid, Hendrik J. Nel, Ranjeny Thomas
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-01-01
Series:Frontiers in Immunology
Subjects:
antigen-specific T cells
tetramers
tetramerization
protocol standardization
flow cytometry
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2018.00008/full

Internet

http://journal.frontiersin.org/article/10.3389/fimmu.2018.00008/full

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