Enrichment Free qPCR for Rapid Identification and Quantification of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i>, and <i>C. upsaliensis</i> in Chicken Meat Samples by a New Couple of Primers
<i>Campylobacter</i> is the main cause of bacterial foodborne disease and poultry meat is the principal source of human infections. Rapid methods for <i>Campylobacter</i> detection are urgently needed to decrease high bacterial prevalence in poultry products. In this study, w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/10/2341 |
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| author | Priya Vizzini Jasmina Vidic Marisa Manzano |
| author_facet | Priya Vizzini Jasmina Vidic Marisa Manzano |
| author_sort | Priya Vizzini |
| collection | DOAJ |
| description | <i>Campylobacter</i> is the main cause of bacterial foodborne disease and poultry meat is the principal source of human infections. Rapid methods for <i>Campylobacter</i> detection are urgently needed to decrease high bacterial prevalence in poultry products. In this study, we developed new primers, CampyPFw and CampyPRv, that target the 16S-23S rRNA genes of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i> and <i>C. upsaliensis.</i> The primers were tested on positive and negative reference strains in pure cultures and in inoculated poultry meat samples before their application in real-time PCR (qPCR) protocol for analyzing chicken meat samples. In parallel, the samples were tested by using the ISO 10272-1:2006 method. The qPCR protocol based on CampyPFw and CampyPRv showed good sensitivity, with the limit of detection of 4.6 × 10<sup>2</sup> cells/mL in chicken samples without enrichment steps. |
| first_indexed | 2024-03-10T06:33:26Z |
| format | Article |
| id | doaj.art-250267958095474c931d46ac8c9865c0 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T06:33:26Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-250267958095474c931d46ac8c9865c02023-11-22T18:14:29ZengMDPI AGFoods2304-81582021-09-011010234110.3390/foods10102341Enrichment Free qPCR for Rapid Identification and Quantification of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i>, and <i>C. upsaliensis</i> in Chicken Meat Samples by a New Couple of PrimersPriya Vizzini0Jasmina Vidic1Marisa Manzano2Dipartimento di Scienze AgroAlimentari, Ambientali e Animali, Università di Udine, 33100 Udine, ItalyAgroParisTech, INRAE, Micalis Institute, Université Paris-Saclay, 78350 Jouy en Josas, FranceDipartimento di Scienze AgroAlimentari, Ambientali e Animali, Università di Udine, 33100 Udine, Italy<i>Campylobacter</i> is the main cause of bacterial foodborne disease and poultry meat is the principal source of human infections. Rapid methods for <i>Campylobacter</i> detection are urgently needed to decrease high bacterial prevalence in poultry products. In this study, we developed new primers, CampyPFw and CampyPRv, that target the 16S-23S rRNA genes of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i> and <i>C. upsaliensis.</i> The primers were tested on positive and negative reference strains in pure cultures and in inoculated poultry meat samples before their application in real-time PCR (qPCR) protocol for analyzing chicken meat samples. In parallel, the samples were tested by using the ISO 10272-1:2006 method. The qPCR protocol based on CampyPFw and CampyPRv showed good sensitivity, with the limit of detection of 4.6 × 10<sup>2</sup> cells/mL in chicken samples without enrichment steps.https://www.mdpi.com/2304-8158/10/10/2341<i>Campylobacter</i> spp.qPCRspiked chicken meatfoodborne pathogens |
| spellingShingle | Priya Vizzini Jasmina Vidic Marisa Manzano Enrichment Free qPCR for Rapid Identification and Quantification of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i>, and <i>C. upsaliensis</i> in Chicken Meat Samples by a New Couple of Primers Foods <i>Campylobacter</i> spp. qPCR spiked chicken meat foodborne pathogens |
| title | Enrichment Free qPCR for Rapid Identification and Quantification of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i>, and <i>C. upsaliensis</i> in Chicken Meat Samples by a New Couple of Primers |
| title_full | Enrichment Free qPCR for Rapid Identification and Quantification of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i>, and <i>C. upsaliensis</i> in Chicken Meat Samples by a New Couple of Primers |
| title_fullStr | Enrichment Free qPCR for Rapid Identification and Quantification of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i>, and <i>C. upsaliensis</i> in Chicken Meat Samples by a New Couple of Primers |
| title_full_unstemmed | Enrichment Free qPCR for Rapid Identification and Quantification of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i>, and <i>C. upsaliensis</i> in Chicken Meat Samples by a New Couple of Primers |
| title_short | Enrichment Free qPCR for Rapid Identification and Quantification of <i>Campylobacter jejuni</i>, <i>C. coli</i>, <i>C. lari</i>, and <i>C. upsaliensis</i> in Chicken Meat Samples by a New Couple of Primers |
| title_sort | enrichment free qpcr for rapid identification and quantification of i campylobacter jejuni i i c coli i i c lari i and i c upsaliensis i in chicken meat samples by a new couple of primers |
| topic | <i>Campylobacter</i> spp. qPCR spiked chicken meat foodborne pathogens |
| url | https://www.mdpi.com/2304-8158/10/10/2341 |
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