Specificity and mechanism of TonB-dependent ferric catecholate uptake by Fiu

Specificity and mechanism of TonB-dependent ferric catecholate uptake by Fiu

We studied the Escherichia coli outer membrane protein Fiu, a presumed transporter of monomeric ferric catecholates, by introducing Cys residues in its surface loops and modifying them with fluorescein maleimide (FM). Fiu-FM bound iron complexes of the tricatecholate siderophore enterobactin (FeEnt)...

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Bibliographic Details
Main Authors: Taihao Yang, Ye Zou, Ho Leung Ng, Ashish Kumar, Salete M. Newton, Phillip E. Klebba
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-03-01
Series:Frontiers in Microbiology
Subjects:
siderophore
catecholate
iron transport
TonB
fluorescent sensor
site-directed mutagenesis
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1355253/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2024.1355253/full

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