Protocol for isolating CD163+ Kupffer cells from human liver resections

Protocol for isolating CD163+ Kupffer cells from human liver resections

Summary: The liver microenvironment contains a wide variety of monocyte and macrophage populations. Here, we present a protocol for the specific isolation of liver-resident macrophages, known as Kupffer cells (KCs), from human liver resections. We describe steps for dissociating human liver tissues,...

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Bibliographic Details
Main Authors: Armando Andres Roca Suarez, Marie-Laure Plissonnier, Maud Michelet, Anaëlle Dubois, Sarah Heintz, Maria Saez-Palma, Marion Delphin, Isabelle Bordes, Jennifer Molle, Audrey Diederichs, Mélanie Rodà, Emmanuel Combe, Léa Mougené, Guillaume Giraud, Xavier Grand, Michel Rivoire, Massimo Levrero, Barbara Testoni, Fabien Zoulim
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005240

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724005240

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