Characterization of Aspartate Kinase from Corynebacterium pekinense and the Critical Site of Arg169
Aspartate kinase (AK) is the key enzyme in the biosynthesis of aspartate-derived amino acids. Recombinant AK was efficiently purified and systematically characterized through analysis under optimal conditions combined with steady-state kinetics study. Homogeneous AK was predicted as a decamer with a...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2015-11-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/1422-0067/16/12/26098 |