Organotypic Slice Culture of the Embryonic Mouse Brain

Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.

Bibliographic Details
Main Authors: James Clegg, Thomas Pratt
Format: Article
Language:English
Published: Bio-protocol LLC 2020-07-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=3674&type=0