Antioxidant PDA-PEG nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral bone
Abstract Accumulating evidence suggests that osteoclastogenesis and angiogenesis in subchondral bone are critical destructive factors in the initiation and progression of osteoarthritis (OA). Herein, methoxypolyethylene glycol amine (mPEG-NH2) modified polydopamine nanoparticles (PDA-PEG NPs) were s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-022-01697-y |
| _version_ | 1798016728723095552 |
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| author | Zhikai Wu Kai Yuan Qian Zhang Jiong Jiong Guo Huilin Yang Feng Zhou |
| author_facet | Zhikai Wu Kai Yuan Qian Zhang Jiong Jiong Guo Huilin Yang Feng Zhou |
| author_sort | Zhikai Wu |
| collection | DOAJ |
| description | Abstract Accumulating evidence suggests that osteoclastogenesis and angiogenesis in subchondral bone are critical destructive factors in the initiation and progression of osteoarthritis (OA). Herein, methoxypolyethylene glycol amine (mPEG-NH2) modified polydopamine nanoparticles (PDA-PEG NPs) were synthesized for treating early OA. The cytotoxicity and reactive oxygen species (ROS) scavenging ability of PDA-PEG NPs were evaluated. The effects of PDA-PEG NPs on osteoclast differentiation and vessel formation were then evaluated. Further, PDA-PEG NPs were administrated to anterior cruciate ligament transection (ACLT)-induced OA mice. Results demonstrated that PDA-PEG NPs had low toxicity both in vitro and in vivo. PDA-PEG NPs could inhibit osteoclastogenesis via regulating nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Moreover, PDA-PEG NPs suppressed osteoclast-related angiogenesis via down-regulating platelet-derived growth factor-BB (PDGF-BB). In vivo, PDA-PEG NPs inhibited subchondral bone resorption and angiogenesis, further rescuing cartilage degradation in OA mice. In conclusion, we demonstrated that PDA-PEG NPs deployment could be a potential therapy for OA. Graphical Abstract |
| first_indexed | 2024-04-11T15:55:25Z |
| format | Article |
| id | doaj.art-2570d090119e4a64b3db5641e7e42304 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T15:55:25Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-2570d090119e4a64b3db5641e7e423042022-12-22T04:15:10ZengBMCJournal of Nanobiotechnology1477-31552022-11-0120111710.1186/s12951-022-01697-yAntioxidant PDA-PEG nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral boneZhikai Wu0Kai Yuan1Qian Zhang2Jiong Jiong Guo3Huilin Yang4Feng Zhou5Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow UniversityShanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow UniversityDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow UniversityDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow UniversityDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow UniversityAbstract Accumulating evidence suggests that osteoclastogenesis and angiogenesis in subchondral bone are critical destructive factors in the initiation and progression of osteoarthritis (OA). Herein, methoxypolyethylene glycol amine (mPEG-NH2) modified polydopamine nanoparticles (PDA-PEG NPs) were synthesized for treating early OA. The cytotoxicity and reactive oxygen species (ROS) scavenging ability of PDA-PEG NPs were evaluated. The effects of PDA-PEG NPs on osteoclast differentiation and vessel formation were then evaluated. Further, PDA-PEG NPs were administrated to anterior cruciate ligament transection (ACLT)-induced OA mice. Results demonstrated that PDA-PEG NPs had low toxicity both in vitro and in vivo. PDA-PEG NPs could inhibit osteoclastogenesis via regulating nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Moreover, PDA-PEG NPs suppressed osteoclast-related angiogenesis via down-regulating platelet-derived growth factor-BB (PDGF-BB). In vivo, PDA-PEG NPs inhibited subchondral bone resorption and angiogenesis, further rescuing cartilage degradation in OA mice. In conclusion, we demonstrated that PDA-PEG NPs deployment could be a potential therapy for OA. Graphical Abstracthttps://doi.org/10.1186/s12951-022-01697-yPDA-PEG NPsOsteoarthritisOsteoclastogenesisAngiogenesisROSPDGF-BB |
| spellingShingle | Zhikai Wu Kai Yuan Qian Zhang Jiong Jiong Guo Huilin Yang Feng Zhou Antioxidant PDA-PEG nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral bone Journal of Nanobiotechnology PDA-PEG NPs Osteoarthritis Osteoclastogenesis Angiogenesis ROS PDGF-BB |
| title | Antioxidant PDA-PEG nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral bone |
| title_full | Antioxidant PDA-PEG nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral bone |
| title_fullStr | Antioxidant PDA-PEG nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral bone |
| title_full_unstemmed | Antioxidant PDA-PEG nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral bone |
| title_short | Antioxidant PDA-PEG nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral bone |
| title_sort | antioxidant pda peg nanoparticles alleviate early osteoarthritis by inhibiting osteoclastogenesis and angiogenesis in subchondral bone |
| topic | PDA-PEG NPs Osteoarthritis Osteoclastogenesis Angiogenesis ROS PDGF-BB |
| url | https://doi.org/10.1186/s12951-022-01697-y |
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