Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a

Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a

Abstract Background Functionally related genes in bacteria are often organized and transcribed as polycistronic transcriptional units. Examples are the fim operon, which codes for biogenesis of type 1 fimbriae in Escherichia coli, and the atp operon, which codes for the FoF1 ATP synthase. We tested...

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Bibliographic Details
Main Authors: Antoine Graffeuil, Julio Guerrero-Castro, Aster Assefa, Bernt Eric Uhlin, David A. Cisneros
Format: Article
Language:English
Published: BMC 2022-07-01
Series:Microbial Cell Factories
Subjects:
Cas12a
CRISPR mutagenesis
Polycistronic operons
Intracellular ATP
 Markerless genome editing
Online Access:https://doi.org/10.1186/s12934-022-01844-y

Internet

https://doi.org/10.1186/s12934-022-01844-y

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