pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA
Replication of influenza viral genomic RNA (vRNA) is catalyzed by viral RNA-dependent RNA polymerase (vRdRP). Complementary RNA (cRNA) is first copied from vRNA, and progeny vRNAs are then amplified from the cRNA. Although vRdRP and viral RNA are minimal requirements, efficient cell-free replication...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2015-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/08939 |
| _version_ | 1811200019639828480 |
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| author | Kenji Sugiyama Atsushi Kawaguchi Mitsuru Okuwaki Kyosuke Nagata |
| author_facet | Kenji Sugiyama Atsushi Kawaguchi Mitsuru Okuwaki Kyosuke Nagata |
| author_sort | Kenji Sugiyama |
| collection | DOAJ |
| description | Replication of influenza viral genomic RNA (vRNA) is catalyzed by viral RNA-dependent RNA polymerase (vRdRP). Complementary RNA (cRNA) is first copied from vRNA, and progeny vRNAs are then amplified from the cRNA. Although vRdRP and viral RNA are minimal requirements, efficient cell-free replication could not be reproduced using only these viral factors. Using a biochemical complementation assay system, we found a novel activity in the nuclear extracts of uninfected cells, designated IREF-2, that allows robust unprimed vRNA synthesis from a cRNA template. IREF-2 was shown to consist of host-derived proteins, pp32 and APRIL. IREF-2 interacts with a free form of vRdRP and preferentially upregulates vRNA synthesis rather than cRNA synthesis. Knockdown experiments indicated that IREF-2 is involved in in vivo viral replication. On the basis of these results and those of previous studies, a plausible role(s) for IREF-2 during the initiation processes of vRNA replication is discussed. |
| first_indexed | 2024-04-12T01:57:46Z |
| format | Article |
| id | doaj.art-2587074cbb2a46cf9c387b222f9b1174 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:57:46Z |
| publishDate | 2015-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-2587074cbb2a46cf9c387b222f9b11742022-12-22T03:52:46ZengeLife Sciences Publications LtdeLife2050-084X2015-10-01410.7554/eLife.08939pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNAKenji Sugiyama0Atsushi Kawaguchi1Mitsuru Okuwaki2Kyosuke Nagata3https://orcid.org/0000-0003-2522-3561Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanDepartment of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanDepartment of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanDepartment of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanReplication of influenza viral genomic RNA (vRNA) is catalyzed by viral RNA-dependent RNA polymerase (vRdRP). Complementary RNA (cRNA) is first copied from vRNA, and progeny vRNAs are then amplified from the cRNA. Although vRdRP and viral RNA are minimal requirements, efficient cell-free replication could not be reproduced using only these viral factors. Using a biochemical complementation assay system, we found a novel activity in the nuclear extracts of uninfected cells, designated IREF-2, that allows robust unprimed vRNA synthesis from a cRNA template. IREF-2 was shown to consist of host-derived proteins, pp32 and APRIL. IREF-2 interacts with a free form of vRdRP and preferentially upregulates vRNA synthesis rather than cRNA synthesis. Knockdown experiments indicated that IREF-2 is involved in in vivo viral replication. On the basis of these results and those of previous studies, a plausible role(s) for IREF-2 during the initiation processes of vRNA replication is discussed.https://elifesciences.org/articles/08939ANP32de novohost factorinfluenzareplicationRNA dependent RNA polymerase |
| spellingShingle | Kenji Sugiyama Atsushi Kawaguchi Mitsuru Okuwaki Kyosuke Nagata pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA eLife ANP32 de novo host factor influenza replication RNA dependent RNA polymerase |
| title | pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA |
| title_full | pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA |
| title_fullStr | pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA |
| title_full_unstemmed | pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA |
| title_short | pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA |
| title_sort | pp32 and april are host cell derived regulators of influenza virus rna synthesis from crna |
| topic | ANP32 de novo host factor influenza replication RNA dependent RNA polymerase |
| url | https://elifesciences.org/articles/08939 |
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