Preservation of <em>Bacillus firmus</em> Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge
The preservation of <em>Bacillus firmus</em> strain 37 cells by lyophilization was evaluated and response surface methodology (RSM) was used to optimize the β-cyclodextrin (β-CD) production by cells immobilized on loofa sponge. Interactions were studied with the variables...
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| Format: | Article |
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MDPI AG
2012-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/8/9476 |
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| author | Cristiane Moriwaki Teresinha Aparecida Guedes Graciette Matioli Sabrina Barbosa de Souza Ferreira Elder James Silva Santos Rúbia Pazzetto |
| author_facet | Cristiane Moriwaki Teresinha Aparecida Guedes Graciette Matioli Sabrina Barbosa de Souza Ferreira Elder James Silva Santos Rúbia Pazzetto |
| author_sort | Cristiane Moriwaki |
| collection | DOAJ |
| description | The preservation of <em>Bacillus firmus</em> strain 37 cells by lyophilization was evaluated and response surface methodology (RSM) was used to optimize the β-cyclodextrin (β-CD) production by cells immobilized on loofa sponge. Interactions were studied with the variables temperature, pH and dextrin concentration using a central composite design (CCD). Immobilization time influence on β-CD production was also investigated. <em>B. firmus</em> strain 37 cells remained viable after one year of storage, showing that the lyophilization is a suitable method for preservation of the microorganism. From the three-dimensional diagrams and contour plots, the best conditions for β-CD production were determined: temperature 60 °C, pH 8, and 18% dextrin. Considering that the amount of dextrin was high, a new assay was carried out, in which dextrin concentrations of 10, 15, and 18% were tested and the temperature of 60 °C and pH 8 were maintained. The results achieved showed very small differences and therefore, for economic reasons, the use of 10% dextrin is suggested. Increasing the immobilization time of cells immobilized on synthetic sponge the β-CD production decreased and did not change for cells immobilized on loofa sponge. The results of this research are important for microorganism preservation and essential in the optimization of the biosynthesis of CD. |
| first_indexed | 2024-04-13T19:22:08Z |
| format | Article |
| id | doaj.art-259b07bc5f7d47ff919740071b3c65cf |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T19:22:08Z |
| publishDate | 2012-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-259b07bc5f7d47ff919740071b3c65cf2022-12-22T02:33:29ZengMDPI AGMolecules1420-30492012-08-011789476948810.3390/molecules17089476Preservation of <em>Bacillus firmus</em> Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa SpongeCristiane MoriwakiTeresinha Aparecida GuedesGraciette MatioliSabrina Barbosa de Souza FerreiraElder James Silva SantosRúbia PazzettoThe preservation of <em>Bacillus firmus</em> strain 37 cells by lyophilization was evaluated and response surface methodology (RSM) was used to optimize the β-cyclodextrin (β-CD) production by cells immobilized on loofa sponge. Interactions were studied with the variables temperature, pH and dextrin concentration using a central composite design (CCD). Immobilization time influence on β-CD production was also investigated. <em>B. firmus</em> strain 37 cells remained viable after one year of storage, showing that the lyophilization is a suitable method for preservation of the microorganism. From the three-dimensional diagrams and contour plots, the best conditions for β-CD production were determined: temperature 60 °C, pH 8, and 18% dextrin. Considering that the amount of dextrin was high, a new assay was carried out, in which dextrin concentrations of 10, 15, and 18% were tested and the temperature of 60 °C and pH 8 were maintained. The results achieved showed very small differences and therefore, for economic reasons, the use of 10% dextrin is suggested. Increasing the immobilization time of cells immobilized on synthetic sponge the β-CD production decreased and did not change for cells immobilized on loofa sponge. The results of this research are important for microorganism preservation and essential in the optimization of the biosynthesis of CD.http://www.mdpi.com/1420-3049/17/8/9476microorganism preservationresponse surface methodologycyclodextrinloofa spongecells immobilization<em>Bacillus firmus</em> |
| spellingShingle | Cristiane Moriwaki Teresinha Aparecida Guedes Graciette Matioli Sabrina Barbosa de Souza Ferreira Elder James Silva Santos Rúbia Pazzetto Preservation of <em>Bacillus firmus</em> Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge Molecules microorganism preservation response surface methodology cyclodextrin loofa sponge cells immobilization <em>Bacillus firmus</em> |
| title | Preservation of <em>Bacillus firmus</em> Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge |
| title_full | Preservation of <em>Bacillus firmus</em> Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge |
| title_fullStr | Preservation of <em>Bacillus firmus</em> Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge |
| title_full_unstemmed | Preservation of <em>Bacillus firmus</em> Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge |
| title_short | Preservation of <em>Bacillus firmus</em> Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge |
| title_sort | preservation of lt em gt bacillus firmus lt em gt strain 37 and optimization of cyclodextrin biosynthesis by cells immobilized on loofa sponge |
| topic | microorganism preservation response surface methodology cyclodextrin loofa sponge cells immobilization <em>Bacillus firmus</em> |
| url | http://www.mdpi.com/1420-3049/17/8/9476 |
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