Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Abstract Background Phytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme development has focused on increasing thermostability to withstand the high temperatures during industrial steam pelleting. Increasing thermosta...

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Bibliographic Details
Main Authors: Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos H. Luna-Flores, Xiaojing Chen, Juhani von Hellens, Stephen Mahler, Robert Speight
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Biotechnology for Biofuels
Subjects:
Phytase
Thermostability
Disulfide bond
Chaperone
Folding
Online Access:https://doi.org/10.1186/s13068-021-01936-8

Internet

https://doi.org/10.1186/s13068-021-01936-8

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