Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli

Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli

Background: The temperature upshift has been widely used as an induction system to produce recombinant proteins (RPs). However, thermoinduction could affect bacterial metabolism, RP production, and RP aggregation. Understanding the structure and functionality of those aggregates, known as inclusion...

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Bibliographic Details
Main Authors: Sara Restrepo-Pineda, Diego Rosiles-Becerril, Angélica B. Vargas-Castillo, Luis P. Ávila-Barrientos, Axel Luviano, Nuria Sánchez-Puig, Enrique García-Hernández, Nestor O. Pérez, Mauricio A. Trujillo-Roldán, Norma A. Valdez-Cruz
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Electronic Journal of Biotechnology
Subjects:
Acetate
Aggregation
Biomass
Bioprocesses
Escherichia coli
Inclusion bodies
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345822000343

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http://www.sciencedirect.com/science/article/pii/S0717345822000343

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