Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli
Background: The temperature upshift has been widely used as an induction system to produce recombinant proteins (RPs). However, thermoinduction could affect bacterial metabolism, RP production, and RP aggregation. Understanding the structure and functionality of those aggregates, known as inclusion...
Main Authors: | Sara Restrepo-Pineda, Diego Rosiles-Becerril, Angélica B. Vargas-Castillo, Luis P. Ávila-Barrientos, Axel Luviano, Nuria Sánchez-Puig, Enrique García-Hernández, Nestor O. Pérez, Mauricio A. Trujillo-Roldán, Norma A. Valdez-Cruz |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2022-09-01
|
Series: | Electronic Journal of Biotechnology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345822000343 |
Similar Items
-
Acetate as substrate for l-malic acid production with Aspergillus oryzae DSM 1863
by: Aline Kövilein, et al.
Published: (2021-02-01) -
Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures
by: Hyeongmin Seo, et al.
Published: (2019-10-01) -
Optimization of the production process for the anticancer lead compound illudin M: process development in stirred tank bioreactors
by: Lillibeth Chaverra-Muñoz, et al.
Published: (2022-07-01) -
Bioprocess optimization : a challenge /
by: Tapobrata, Panda -
Research Upregulation of CD23 (FcεRII) Expression in Human Airway Smooth Muscle Cells (huASMC) in Response to IL-4, GM-CSF, and IL-4/GM-CSF
by: Lew D Betty, et al.
Published: (2005-05-01)