Transmigration of Trypanosoma brucei across an in vitro blood-cerebrospinal fluid barrier
Summary: Trypanosoma brucei is the causative agent of human African trypanosomiasis. The parasite transmigrates from blood vessels across the choroid plexus epithelium to enter the central nervous system, a process that leads to the manifestation of second stage sleeping sickness. Using an in vitro...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422200284X |
| _version_ | 1819079043230728192 |
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| author | Annika Speidel Marianne Theile Lena Pfeiffer Alexander Herrmann Katherine Figarella Hiroshi Ishikawa Christian Schwerk Horst Schroten Michael Duszenko Stefan Mogk |
| author_facet | Annika Speidel Marianne Theile Lena Pfeiffer Alexander Herrmann Katherine Figarella Hiroshi Ishikawa Christian Schwerk Horst Schroten Michael Duszenko Stefan Mogk |
| author_sort | Annika Speidel |
| collection | DOAJ |
| description | Summary: Trypanosoma brucei is the causative agent of human African trypanosomiasis. The parasite transmigrates from blood vessels across the choroid plexus epithelium to enter the central nervous system, a process that leads to the manifestation of second stage sleeping sickness. Using an in vitro model of the blood-cerebrospinal fluid barrier, we investigated the mechanism of the transmigration process. For this, a monolayer of human choroid plexus papilloma cells was cultivated on a permeable membrane that mimics the basal lamina underlying the choroid plexus epithelial cells. Plexus cells polarize and interconnect forming tight junctions. Deploying different T. brucei brucei strains, we observed that geometry and motility are important for tissue invasion. Using fluorescent microscopy, the parasite’s moving was visualized between plexus epithelial cells. The presented model provides a simple tool to screen trypanosome libraries for their ability to infect cerebrospinal fluid or to test the impact of chemical substances on transmigration. |
| first_indexed | 2024-12-21T19:22:43Z |
| format | Article |
| id | doaj.art-260bc408a281465ea274f0d652b99ec5 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-21T19:22:43Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-260bc408a281465ea274f0d652b99ec52022-12-21T18:52:54ZengElsevieriScience2589-00422022-04-01254104014Transmigration of Trypanosoma brucei across an in vitro blood-cerebrospinal fluid barrierAnnika Speidel0Marianne Theile1Lena Pfeiffer2Alexander Herrmann3Katherine Figarella4Hiroshi Ishikawa5Christian Schwerk6Horst Schroten7Michael Duszenko8Stefan Mogk9Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, GermanyInterfaculty Institute of Biochemistry, University of Tübingen, Tübingen, GermanyInterfaculty Institute of Biochemistry, University of Tübingen, Tübingen, GermanyInterfaculty Institute of Biochemistry, University of Tübingen, Tübingen, GermanyDepartment of Neurophysiology, University of Tübingen, Tübingen, GermanyLaboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanPediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, Mannheim, GermanyPediatric Infectious Diseases, Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, Mannheim, GermanyDepartment of Neurophysiology, University of Tübingen, Tübingen, GermanyInterfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany; Corresponding authorSummary: Trypanosoma brucei is the causative agent of human African trypanosomiasis. The parasite transmigrates from blood vessels across the choroid plexus epithelium to enter the central nervous system, a process that leads to the manifestation of second stage sleeping sickness. Using an in vitro model of the blood-cerebrospinal fluid barrier, we investigated the mechanism of the transmigration process. For this, a monolayer of human choroid plexus papilloma cells was cultivated on a permeable membrane that mimics the basal lamina underlying the choroid plexus epithelial cells. Plexus cells polarize and interconnect forming tight junctions. Deploying different T. brucei brucei strains, we observed that geometry and motility are important for tissue invasion. Using fluorescent microscopy, the parasite’s moving was visualized between plexus epithelial cells. The presented model provides a simple tool to screen trypanosome libraries for their ability to infect cerebrospinal fluid or to test the impact of chemical substances on transmigration.http://www.sciencedirect.com/science/article/pii/S258900422200284XMicrobiologyParasitologyBioengineering |
| spellingShingle | Annika Speidel Marianne Theile Lena Pfeiffer Alexander Herrmann Katherine Figarella Hiroshi Ishikawa Christian Schwerk Horst Schroten Michael Duszenko Stefan Mogk Transmigration of Trypanosoma brucei across an in vitro blood-cerebrospinal fluid barrier iScience Microbiology Parasitology Bioengineering |
| title | Transmigration of Trypanosoma brucei across an in vitro blood-cerebrospinal fluid barrier |
| title_full | Transmigration of Trypanosoma brucei across an in vitro blood-cerebrospinal fluid barrier |
| title_fullStr | Transmigration of Trypanosoma brucei across an in vitro blood-cerebrospinal fluid barrier |
| title_full_unstemmed | Transmigration of Trypanosoma brucei across an in vitro blood-cerebrospinal fluid barrier |
| title_short | Transmigration of Trypanosoma brucei across an in vitro blood-cerebrospinal fluid barrier |
| title_sort | transmigration of trypanosoma brucei across an in vitro blood cerebrospinal fluid barrier |
| topic | Microbiology Parasitology Bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S258900422200284X |
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