Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates
Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, wit...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | EBioMedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396416304431 |
| _version_ | 1819077152454213632 |
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| author | Xiao-Feng Li Hao-Long Dong Xing-Yao Huang Ye-Feng Qiu Hong-Jiang Wang Yong-Qiang Deng Na-Na Zhang Qing Ye Hui Zhao Zhong-Yu Liu Hang Fan Xiao-Ping An Shi-Hui Sun Bo Gao Yun-Zhi Fa Yi-Gang Tong Fu-Chun Zhang George F. Gao Wu-Chun Cao Pei-Yong Shi Cheng-Feng Qin |
| author_facet | Xiao-Feng Li Hao-Long Dong Xing-Yao Huang Ye-Feng Qiu Hong-Jiang Wang Yong-Qiang Deng Na-Na Zhang Qing Ye Hui Zhao Zhong-Yu Liu Hang Fan Xiao-Ping An Shi-Hui Sun Bo Gao Yun-Zhi Fa Yi-Gang Tong Fu-Chun Zhang George F. Gao Wu-Chun Cao Pei-Yong Shi Cheng-Feng Qin |
| author_sort | Xiao-Feng Li |
| collection | DOAJ |
| description | Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, with robust excretion of ZIKV RNA in urine, saliva, and lacrimal fluid. Necropsy of two infected animals revealed that systematic infections involving central nervous system and visceral organs were established at the acute phrase. ZIKV initially targeted the intestinal tracts, spleen, and parotid glands, and retained in spleen and lymph nodes till 10 days post infection. ZIKV-specific immune responses were readily induced in all inoculated animals. The non-human primate model described here provides a valuable platform to study ZIKV pathogenesis and to evaluate vaccine and therapeutics. |
| first_indexed | 2024-12-21T18:52:39Z |
| format | Article |
| id | doaj.art-2620f56e14a84587b55ae54032d296f6 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-21T18:52:39Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-2620f56e14a84587b55ae54032d296f62022-12-21T18:53:42ZengElsevierEBioMedicine2352-39642016-10-0112C17017710.1016/j.ebiom.2016.09.022Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human PrimatesXiao-Feng Li0Hao-Long Dong1Xing-Yao Huang2Ye-Feng Qiu3Hong-Jiang Wang4Yong-Qiang Deng5Na-Na Zhang6Qing Ye7Hui Zhao8Zhong-Yu Liu9Hang Fan10Xiao-Ping An11Shi-Hui Sun12Bo Gao13Yun-Zhi Fa14Yi-Gang Tong15Fu-Chun Zhang16George F. Gao17Wu-Chun Cao18Pei-Yong Shi19Cheng-Feng Qin20State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaLaboratory Animal Center, Academy of Military Medical Science, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaLaboratory Animal Center, Academy of Military Medical Science, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaGuangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaDepartment of Biochemistry and Molecular Biology, Department of Pharmacology and Toxicology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555, USAState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaAnimal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, with robust excretion of ZIKV RNA in urine, saliva, and lacrimal fluid. Necropsy of two infected animals revealed that systematic infections involving central nervous system and visceral organs were established at the acute phrase. ZIKV initially targeted the intestinal tracts, spleen, and parotid glands, and retained in spleen and lymph nodes till 10 days post infection. ZIKV-specific immune responses were readily induced in all inoculated animals. The non-human primate model described here provides a valuable platform to study ZIKV pathogenesis and to evaluate vaccine and therapeutics.http://www.sciencedirect.com/science/article/pii/S2352396416304431Zika virusNon-human primate modelTarget organLacrimal fluid |
| spellingShingle | Xiao-Feng Li Hao-Long Dong Xing-Yao Huang Ye-Feng Qiu Hong-Jiang Wang Yong-Qiang Deng Na-Na Zhang Qing Ye Hui Zhao Zhong-Yu Liu Hang Fan Xiao-Ping An Shi-Hui Sun Bo Gao Yun-Zhi Fa Yi-Gang Tong Fu-Chun Zhang George F. Gao Wu-Chun Cao Pei-Yong Shi Cheng-Feng Qin Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates EBioMedicine Zika virus Non-human primate model Target organ Lacrimal fluid |
| title | Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates |
| title_full | Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates |
| title_fullStr | Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates |
| title_full_unstemmed | Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates |
| title_short | Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates |
| title_sort | characterization of a 2016 clinical isolate of zika virus in non human primates |
| topic | Zika virus Non-human primate model Target organ Lacrimal fluid |
| url | http://www.sciencedirect.com/science/article/pii/S2352396416304431 |
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