Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells

Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells

Summary: Interferon regulatory factor 4 (IRF4) is a transcription factor that regulates normal and malignant immune cell development and is implicated in multiple myeloma pathogenesis. This protocol describes the use of combined cell surface and intranuclear staining with fluorescent antibodies to m...

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Bibliographic Details
Main Authors: Ashni A. Vora, Phoebe K. Mondala, Caitlin Costello, A. Robert MacLeod, Leslie A. Crews
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cancer
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002720

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http://www.sciencedirect.com/science/article/pii/S2666166721002720

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