Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells
Summary: Interferon regulatory factor 4 (IRF4) is a transcription factor that regulates normal and malignant immune cell development and is implicated in multiple myeloma pathogenesis. This protocol describes the use of combined cell surface and intranuclear staining with fluorescent antibodies to m...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721002720 |
| _version_ | 1818449076754055168 |
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| author | Ashni A. Vora Phoebe K. Mondala Caitlin Costello A. Robert MacLeod Leslie A. Crews |
| author_facet | Ashni A. Vora Phoebe K. Mondala Caitlin Costello A. Robert MacLeod Leslie A. Crews |
| author_sort | Ashni A. Vora |
| collection | DOAJ |
| description | Summary: Interferon regulatory factor 4 (IRF4) is a transcription factor that regulates normal and malignant immune cell development and is implicated in multiple myeloma pathogenesis. This protocol describes the use of combined cell surface and intranuclear staining with fluorescent antibodies to measure IRF4 protein expression within myeloma and normal immune cells. IRF4 protein quantification may provide a valuable prognostic tool to predict disease severity and sensitivity to IRF4-targeted therapies. This flow-cytometry-based procedure could also be rapidly translated into a clinically compatible assay.For complete details on the use and execution of this protocol, please refer to Mondala et al. (2021). |
| first_indexed | 2024-12-14T20:29:40Z |
| format | Article |
| id | doaj.art-262649648a7f493f880dcdf716246095 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-14T20:29:40Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-262649648a7f493f880dcdf7162460952022-12-21T22:48:33ZengElsevierSTAR Protocols2666-16672021-06-0122100565Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cellsAshni A. Vora0Phoebe K. Mondala1Caitlin Costello2A. Robert MacLeod3Leslie A. Crews4Division of Regenerative Medicine, Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USADivision of Regenerative Medicine, Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USADivision of Blood and Marrow Transplantation, Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA; Moores Cancer Center at University of California, San Diego, La Jolla, CA 92093, USAIonis Pharmaceuticals, Carlsbad, CA 92008, USADivision of Regenerative Medicine, Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA; Moores Cancer Center at University of California, San Diego, La Jolla, CA 92093, USA; Corresponding authorSummary: Interferon regulatory factor 4 (IRF4) is a transcription factor that regulates normal and malignant immune cell development and is implicated in multiple myeloma pathogenesis. This protocol describes the use of combined cell surface and intranuclear staining with fluorescent antibodies to measure IRF4 protein expression within myeloma and normal immune cells. IRF4 protein quantification may provide a valuable prognostic tool to predict disease severity and sensitivity to IRF4-targeted therapies. This flow-cytometry-based procedure could also be rapidly translated into a clinically compatible assay.For complete details on the use and execution of this protocol, please refer to Mondala et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721002720CancerCell isolationFlow Cytometry/Mass CytometryImmunologyStem Cells |
| spellingShingle | Ashni A. Vora Phoebe K. Mondala Caitlin Costello A. Robert MacLeod Leslie A. Crews Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells STAR Protocols Cancer Cell isolation Flow Cytometry/Mass Cytometry Immunology Stem Cells |
| title | Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells |
| title_full | Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells |
| title_fullStr | Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells |
| title_full_unstemmed | Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells |
| title_short | Sensitive intranuclear flow cytometric quantification of IRF4 protein in multiple myeloma and normal human hematopoietic cells |
| title_sort | sensitive intranuclear flow cytometric quantification of irf4 protein in multiple myeloma and normal human hematopoietic cells |
| topic | Cancer Cell isolation Flow Cytometry/Mass Cytometry Immunology Stem Cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721002720 |
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