Integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish (Procambarus clarkii) muscle under cold stress
Related research findings indicated that the hardness of the tail meat from red swamp crayfish (Procambarus clarkii) increased when responding to cold stress during the transportation. However, the effect of low temperature on crayfish muscle was still at the phenotype level, there were few studies...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-05-01
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| Series: | Food Science of Animal Products |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSAP.2023.9240007 |
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| author | Yuqing Lei Ying Gao Xuehong Li Xiaoying Luo Lan Wang Wenjin Wu Guangquan Xiong Shang Chu Shugang Li |
| author_facet | Yuqing Lei Ying Gao Xuehong Li Xiaoying Luo Lan Wang Wenjin Wu Guangquan Xiong Shang Chu Shugang Li |
| author_sort | Yuqing Lei |
| collection | DOAJ |
| description | Related research findings indicated that the hardness of the tail meat from red swamp crayfish (Procambarus clarkii) increased when responding to cold stress during the transportation. However, the effect of low temperature on crayfish muscle was still at the phenotype level, there were few studies on the molecular mechanism of crayfish muscle response to cold stress. The effect of cold stress on the tail meat of crayfish during simulated transportation (control and low temperature stress for 12 h (LT_12), 24 h (LT_24) and 36 h (LT_36) at 4 ℃) were investigated by integrated transcriptome and proteomics. The results showed that the hardness of crayfish meat increased after cold stress. Gene ontology (GO) analysis showed that differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) of crayfish coping with cold stress were mainly involved in metabolism and glycolysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic analysis found that the metabolic response to cold stress included changes in amino acids such as valine and isoleucine. Low temperature activated glycolysis and amino acid metabolism pathway as well as peroxisome pathway to maintain body balance. The significant increase in the expression of cytoskeletal protein-actin related genes such as β-actin and ACT1 might cause the increase of muscle hardness under stress. |
| first_indexed | 2024-03-07T21:18:35Z |
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| id | doaj.art-264c190a8ba84b5086756f28a1f517c5 |
| institution | Directory Open Access Journal |
| issn | 2958-4124 2958-3780 |
| language | English |
| last_indexed | 2024-03-07T21:18:35Z |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science of Animal Products |
| spelling | doaj.art-264c190a8ba84b5086756f28a1f517c52024-02-27T15:28:39ZengTsinghua University PressFood Science of Animal Products2958-41242958-37802023-05-0111924000710.26599/FSAP.2023.9240007Integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish (Procambarus clarkii) muscle under cold stressYuqing Lei0Ying Gao1Xuehong Li2Xiaoying Luo3Lan Wang4Wenjin Wu5Guangquan Xiong6Shang Chu7Shugang Li8Engineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, ChinaEngineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, ChinaKey Laboratory of Fermentation Engineering, Ministry of Education, School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, ChinaKey Laboratory of Fermentation Engineering, Ministry of Education, School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, ChinaInstitute for Farm Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Science, Wuhan 430064, ChinaInstitute for Farm Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Science, Wuhan 430064, ChinaInstitute for Farm Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Science, Wuhan 430064, ChinaKey Laboratory of Fermentation Engineering, Ministry of Education, School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, ChinaEngineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, ChinaRelated research findings indicated that the hardness of the tail meat from red swamp crayfish (Procambarus clarkii) increased when responding to cold stress during the transportation. However, the effect of low temperature on crayfish muscle was still at the phenotype level, there were few studies on the molecular mechanism of crayfish muscle response to cold stress. The effect of cold stress on the tail meat of crayfish during simulated transportation (control and low temperature stress for 12 h (LT_12), 24 h (LT_24) and 36 h (LT_36) at 4 ℃) were investigated by integrated transcriptome and proteomics. The results showed that the hardness of crayfish meat increased after cold stress. Gene ontology (GO) analysis showed that differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) of crayfish coping with cold stress were mainly involved in metabolism and glycolysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic analysis found that the metabolic response to cold stress included changes in amino acids such as valine and isoleucine. Low temperature activated glycolysis and amino acid metabolism pathway as well as peroxisome pathway to maintain body balance. The significant increase in the expression of cytoskeletal protein-actin related genes such as β-actin and ACT1 might cause the increase of muscle hardness under stress.https://www.sciopen.com/article/10.26599/FSAP.2023.9240007procambarus clarkiitranscriptomeproteomicscold stressglycolysiscytoskeleton structure |
| spellingShingle | Yuqing Lei Ying Gao Xuehong Li Xiaoying Luo Lan Wang Wenjin Wu Guangquan Xiong Shang Chu Shugang Li Integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish (Procambarus clarkii) muscle under cold stress Food Science of Animal Products procambarus clarkii transcriptome proteomics cold stress glycolysis cytoskeleton structure |
| title | Integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish (Procambarus clarkii) muscle under cold stress |
| title_full | Integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish (Procambarus clarkii) muscle under cold stress |
| title_fullStr | Integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish (Procambarus clarkii) muscle under cold stress |
| title_full_unstemmed | Integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish (Procambarus clarkii) muscle under cold stress |
| title_short | Integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish (Procambarus clarkii) muscle under cold stress |
| title_sort | integrating transcriptomic and proteomics revealed the response mechanism of red swamp crayfish procambarus clarkii muscle under cold stress |
| topic | procambarus clarkii transcriptome proteomics cold stress glycolysis cytoskeleton structure |
| url | https://www.sciopen.com/article/10.26599/FSAP.2023.9240007 |
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