Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein Synthesis
A cell-free system from Plasmodium falciparum able to translate endogenous mRNA was used to determine the effect of artemisinin, chloroquine and primaquine on the protein synthesis mechanism of the parasite. The antimalarial drugs did not inhibit the incorporation of [³H] methionine into parasite pr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2002-04-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000300018 |
| _version_ | 1827845035363663872 |
|---|---|
| author | Ana Ferreras Ledia Triana Erlinda Sánchez Flor Herrera |
| author_facet | Ana Ferreras Ledia Triana Erlinda Sánchez Flor Herrera |
| author_sort | Ana Ferreras |
| collection | DOAJ |
| description | A cell-free system from Plasmodium falciparum able to translate endogenous mRNA was used to determine the effect of artemisinin, chloroquine and primaquine on the protein synthesis mechanism of the parasite. The antimalarial drugs did not inhibit the incorporation of [³H] methionine into parasite proteins even at concentrations higher than the ones found to strongly inhibit the parasite growth. Results clearly indicate that these compounds do not have a direct effect on protein synthesis activity of P. falciparum coded by endogenous mRNA. |
| first_indexed | 2024-03-12T08:51:41Z |
| format | Article |
| id | doaj.art-265387bab75a491a9f4fed5d7dab2b75 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:51:41Z |
| publishDate | 2002-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-265387bab75a491a9f4fed5d7dab2b752023-09-02T16:17:47ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602002-04-0197337738010.1590/S0074-02762002000300018Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein SynthesisAna FerrerasLedia TrianaErlinda SánchezFlor HerreraA cell-free system from Plasmodium falciparum able to translate endogenous mRNA was used to determine the effect of artemisinin, chloroquine and primaquine on the protein synthesis mechanism of the parasite. The antimalarial drugs did not inhibit the incorporation of [³H] methionine into parasite proteins even at concentrations higher than the ones found to strongly inhibit the parasite growth. Results clearly indicate that these compounds do not have a direct effect on protein synthesis activity of P. falciparum coded by endogenous mRNA.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000300018Plasmodium falciparumprotein synthesiscell free translationantimalarial drugs |
| spellingShingle | Ana Ferreras Ledia Triana Erlinda Sánchez Flor Herrera Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein Synthesis Memorias do Instituto Oswaldo Cruz Plasmodium falciparum protein synthesis cell free translation antimalarial drugs |
| title | Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein Synthesis |
| title_full | Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein Synthesis |
| title_fullStr | Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein Synthesis |
| title_full_unstemmed | Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein Synthesis |
| title_short | Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein Synthesis |
| title_sort | effect of antimalarial drugs on plasmodia cell free protein synthesis |
| topic | Plasmodium falciparum protein synthesis cell free translation antimalarial drugs |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000300018 |
| work_keys_str_mv | AT anaferreras effectofantimalarialdrugsonplasmodiacellfreeproteinsynthesis AT lediatriana effectofantimalarialdrugsonplasmodiacellfreeproteinsynthesis AT erlindasanchez effectofantimalarialdrugsonplasmodiacellfreeproteinsynthesis AT florherrera effectofantimalarialdrugsonplasmodiacellfreeproteinsynthesis |