Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome

Basal phosphoinositide-dependent phospholipase C (PI-PLC) activity controls gene expression in Arabidopsis suspension cells and seedlings. PI-PLC catalyzes the production of phosphorylated inositol and diacylglycerol (DAG) from phosphoinositides. It is not known how PI-PLC regulates the transcriptom...

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Main Authors: Eric eRuelland, Igor ePokotylo, Catherine eCantrel, Nabila eDjafi, Anne eRepellin, Alain eZachowski
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-11-01
Series:Frontiers in Plant Science
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00608/full
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author Eric eRuelland
Eric eRuelland
Eric eRuelland
Igor ePokotylo
Igor ePokotylo
Catherine eCantrel
Catherine eCantrel
Nabila eDjafi
Anne eRepellin
Alain eZachowski
Alain eZachowski
Alain eZachowski
author_facet Eric eRuelland
Eric eRuelland
Eric eRuelland
Igor ePokotylo
Igor ePokotylo
Catherine eCantrel
Catherine eCantrel
Nabila eDjafi
Anne eRepellin
Alain eZachowski
Alain eZachowski
Alain eZachowski
author_sort Eric eRuelland
collection DOAJ
description Basal phosphoinositide-dependent phospholipase C (PI-PLC) activity controls gene expression in Arabidopsis suspension cells and seedlings. PI-PLC catalyzes the production of phosphorylated inositol and diacylglycerol (DAG) from phosphoinositides. It is not known how PI-PLC regulates the transcriptome although the action of DAG-kinase (DGK) on DAG immediately downstream from PI-PLC is responsible for some of the regulation. We previously established a list of genes whose expression is affected in the presence of PI-PLC inhibitors. Here this list of genes was used as a signature in similarity searches of curated plant hormone response transcriptome data. The strongest correlations obtained with the inhibited PI-PLC signature were with salicylic acid (SA) treatments. We confirm here that in Arabidopsis suspension cells SA treatment leads to an increase in phosphoinositides, then demonstrate that SA leads to a significant 20% decrease in phosphatidic acid, indicative of a decrease in PI-PLC products. Previous sets of microarray data were re-assessed. The SA response of one set of genes was dependent on phosphoinositides. Alterations in the levels of a second set of genes, mostly SA-repressed genes, could be related to decreases in PI-PLC products that occur in response to SA action. Together, the two groups of genes comprise at least 40% of all SA-responsive genes. Overall these two groups of genes are distinct in the functional categories of the proteins they encode, their promoter cis-elements and their regulation by DGK or phospholipase D. SA-regulated genes dependent on phosphoinositides are typical SA response genes while those with an SA response that is possibly dependent on PI-PLC products are less SA-specific. We propose a model in which SA inhibits PI-PLC activity and alters levels of PI-PLC products and substrates, thereby regulating gene expression divergently.
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spelling doaj.art-266137da4dee4513b25bbfdc7178cb8c2022-12-21T22:59:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2014-11-01510.3389/fpls.2014.00608104890Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptomeEric eRuelland0Eric eRuelland1Eric eRuelland2Igor ePokotylo3Igor ePokotylo4Catherine eCantrel5Catherine eCantrel6Nabila eDjafi7Anne eRepellin8Alain eZachowski9Alain eZachowski10Alain eZachowski11Centre National de la Recherche ScientifiqueUniversité Paris-Est CréteilCentre National de la Recherche ScientifiqueUniversité Paris-Est CréteilNational academy of Sciences of UkraineCentre National de la Recherche ScientifiqueCentre National de la Recherche ScientifiqueCentre National de la Recherche ScientifiqueUniversité Paris-Est CréteilCentre National de la Recherche ScientifiqueUniversité Paris-Est CréteilCentre National de la Recherche ScientifiqueBasal phosphoinositide-dependent phospholipase C (PI-PLC) activity controls gene expression in Arabidopsis suspension cells and seedlings. PI-PLC catalyzes the production of phosphorylated inositol and diacylglycerol (DAG) from phosphoinositides. It is not known how PI-PLC regulates the transcriptome although the action of DAG-kinase (DGK) on DAG immediately downstream from PI-PLC is responsible for some of the regulation. We previously established a list of genes whose expression is affected in the presence of PI-PLC inhibitors. Here this list of genes was used as a signature in similarity searches of curated plant hormone response transcriptome data. The strongest correlations obtained with the inhibited PI-PLC signature were with salicylic acid (SA) treatments. We confirm here that in Arabidopsis suspension cells SA treatment leads to an increase in phosphoinositides, then demonstrate that SA leads to a significant 20% decrease in phosphatidic acid, indicative of a decrease in PI-PLC products. Previous sets of microarray data were re-assessed. The SA response of one set of genes was dependent on phosphoinositides. Alterations in the levels of a second set of genes, mostly SA-repressed genes, could be related to decreases in PI-PLC products that occur in response to SA action. Together, the two groups of genes comprise at least 40% of all SA-responsive genes. Overall these two groups of genes are distinct in the functional categories of the proteins they encode, their promoter cis-elements and their regulation by DGK or phospholipase D. SA-regulated genes dependent on phosphoinositides are typical SA response genes while those with an SA response that is possibly dependent on PI-PLC products are less SA-specific. We propose a model in which SA inhibits PI-PLC activity and alters levels of PI-PLC products and substrates, thereby regulating gene expression divergently.http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00608/fullArabidopsisDiacylglycerol KinaseSalicylic Acidlipid signalingphospholipase Ctrancriptomic
spellingShingle Eric eRuelland
Eric eRuelland
Eric eRuelland
Igor ePokotylo
Igor ePokotylo
Catherine eCantrel
Catherine eCantrel
Nabila eDjafi
Anne eRepellin
Alain eZachowski
Alain eZachowski
Alain eZachowski
Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome
Frontiers in Plant Science
Arabidopsis
Diacylglycerol Kinase
Salicylic Acid
lipid signaling
phospholipase C
trancriptomic
title Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome
title_full Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome
title_fullStr Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome
title_full_unstemmed Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome
title_short Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome
title_sort salicylic acid modulates levels of phosphoinositide dependent phospholipase c substrates and products to remodel the arabidopsis suspension cell transcriptome
topic Arabidopsis
Diacylglycerol Kinase
Salicylic Acid
lipid signaling
phospholipase C
trancriptomic
url http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00608/full
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