Optimization of Main Ingredient Ratio, Metabolomics Analysis, and Antioxidant Activity Analysis of Lycopene-Enriched Compound Fruit Wine

To find the optimal main ingredient ratio of compound fruit wine for enriching the varieties of lycopene-enriched fruit products and improving their economic value, fuzzy mathematics sensory evaluation and the D-optimal mixture design were considered. Under the main ingredient ratios of tomato juice...

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Main Authors: Kunyi Liu, Xiangyu Liu, Teng Wang, Qi Wang, Lei Feng, Rui Su, Meng Zhang, Bin Xu, Fei Chen, Pingping Li
Format: Article
Language:English
Published: MDPI AG 2023-06-01
Series:Fermentation
Subjects:
Online Access:https://www.mdpi.com/2311-5637/9/7/591
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author Kunyi Liu
Xiangyu Liu
Teng Wang
Qi Wang
Lei Feng
Rui Su
Meng Zhang
Bin Xu
Fei Chen
Pingping Li
author_facet Kunyi Liu
Xiangyu Liu
Teng Wang
Qi Wang
Lei Feng
Rui Su
Meng Zhang
Bin Xu
Fei Chen
Pingping Li
author_sort Kunyi Liu
collection DOAJ
description To find the optimal main ingredient ratio of compound fruit wine for enriching the varieties of lycopene-enriched fruit products and improving their economic value, fuzzy mathematics sensory evaluation and the D-optimal mixture design were considered. Under the main ingredient ratios of tomato juice, papaya juice, carrot juice, and gac fruit juice of 27.2%, 27.5%, 10.0%, and 35.3%, respectively, a clear and transparent compound fruit wine with a full-bodied fruit and wine aroma and mellow taste can be obtained. Meanwhile, a total of 406 metabolites were identified in the compound fruit wine, which were classified into nine superclasses including lipids and lipid-like molecules (150), organic acids and derivatives (69), and others. The relative levels of 54 metabolites after optimization were decreased significantly (VIP > 1.0, <i>p</i> < 0.05, FC < 0.5), while the relative levels of 106 metabolites including lycopene and (13Z)-lycopene were increased significantly (VIP > 1.0, <i>p</i> < 0.05, FC > 2). Furthermore, the EC<sub>50</sub> values of this compound fruit wine after optimization of the main ingredient ratio for scavenging ABTS<sup>+</sup>·, DPPH·, O<sub>2</sub><sup>−</sup>·, and ·OH were 78.62%, 57.74%, 42.85%, and 59.91%, respectively. Together, a compound fruit wine rich in lycopene with antioxidant activities was manufactured, which has application potential in the development of functional foods.
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spelling doaj.art-2666a796c09c4e8397ac64a2362a9b2c2023-11-18T19:15:34ZengMDPI AGFermentation2311-56372023-06-019759110.3390/fermentation9070591Optimization of Main Ingredient Ratio, Metabolomics Analysis, and Antioxidant Activity Analysis of Lycopene-Enriched Compound Fruit WineKunyi Liu0Xiangyu Liu1Teng Wang2Qi Wang3Lei Feng4Rui Su5Meng Zhang6Bin Xu7Fei Chen8Pingping Li9College of Wuliangye Technology and Food Engineering, Yibin Vocational and Technical College, Yibin 644003, ChinaCollege of Agronomy and Biotechnology, National-Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Wuliangye Technology and Food Engineering, Yibin Vocational and Technical College, Yibin 644003, ChinaCollege of Agronomy and Biotechnology, National-Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming 650201, ChinaSericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661100, ChinaCollege of Life and Environment Science, Huangshan University, Huangshan 245041, ChinaLuzhou Greenland Liquor Co., Ltd., Luzhou 646000, ChinaCollege of Life and Environment Science, Huangshan University, Huangshan 245041, ChinaSericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661100, ChinaTo find the optimal main ingredient ratio of compound fruit wine for enriching the varieties of lycopene-enriched fruit products and improving their economic value, fuzzy mathematics sensory evaluation and the D-optimal mixture design were considered. Under the main ingredient ratios of tomato juice, papaya juice, carrot juice, and gac fruit juice of 27.2%, 27.5%, 10.0%, and 35.3%, respectively, a clear and transparent compound fruit wine with a full-bodied fruit and wine aroma and mellow taste can be obtained. Meanwhile, a total of 406 metabolites were identified in the compound fruit wine, which were classified into nine superclasses including lipids and lipid-like molecules (150), organic acids and derivatives (69), and others. The relative levels of 54 metabolites after optimization were decreased significantly (VIP > 1.0, <i>p</i> < 0.05, FC < 0.5), while the relative levels of 106 metabolites including lycopene and (13Z)-lycopene were increased significantly (VIP > 1.0, <i>p</i> < 0.05, FC > 2). Furthermore, the EC<sub>50</sub> values of this compound fruit wine after optimization of the main ingredient ratio for scavenging ABTS<sup>+</sup>·, DPPH·, O<sub>2</sub><sup>−</sup>·, and ·OH were 78.62%, 57.74%, 42.85%, and 59.91%, respectively. Together, a compound fruit wine rich in lycopene with antioxidant activities was manufactured, which has application potential in the development of functional foods.https://www.mdpi.com/2311-5637/9/7/591antioxidant activitycompound fruit winefuzzy mathematicslycopenesensory score
spellingShingle Kunyi Liu
Xiangyu Liu
Teng Wang
Qi Wang
Lei Feng
Rui Su
Meng Zhang
Bin Xu
Fei Chen
Pingping Li
Optimization of Main Ingredient Ratio, Metabolomics Analysis, and Antioxidant Activity Analysis of Lycopene-Enriched Compound Fruit Wine
Fermentation
antioxidant activity
compound fruit wine
fuzzy mathematics
lycopene
sensory score
title Optimization of Main Ingredient Ratio, Metabolomics Analysis, and Antioxidant Activity Analysis of Lycopene-Enriched Compound Fruit Wine
title_full Optimization of Main Ingredient Ratio, Metabolomics Analysis, and Antioxidant Activity Analysis of Lycopene-Enriched Compound Fruit Wine
title_fullStr Optimization of Main Ingredient Ratio, Metabolomics Analysis, and Antioxidant Activity Analysis of Lycopene-Enriched Compound Fruit Wine
title_full_unstemmed Optimization of Main Ingredient Ratio, Metabolomics Analysis, and Antioxidant Activity Analysis of Lycopene-Enriched Compound Fruit Wine
title_short Optimization of Main Ingredient Ratio, Metabolomics Analysis, and Antioxidant Activity Analysis of Lycopene-Enriched Compound Fruit Wine
title_sort optimization of main ingredient ratio metabolomics analysis and antioxidant activity analysis of lycopene enriched compound fruit wine
topic antioxidant activity
compound fruit wine
fuzzy mathematics
lycopene
sensory score
url https://www.mdpi.com/2311-5637/9/7/591
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