Construction of an IS-Free Corynebacterium glutamicum ATCC 13 032 Chassis Strain and Random Mutagenesis Using the Endogenous ISCg1 Transposase

Construction of an IS-Free Corynebacterium glutamicum ATCC 13 032 Chassis Strain and Random Mutagenesis Using the Endogenous ISCg1 Transposase

Mobile genetic elements (MGEs) contribute to instability of the host genome and plasmids. Previously, removal of the prophages in the industrial amino acid producer Corynebacterium glutamicum ATCC 13 032 resulted in strain MB001 which showed better survival under stress conditions and increased tran...

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Bibliographic Details
Main Authors: Marten Linder , Markus Haak , Angela Botes , Jörn Kalinowski , Christian Rückert 
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-12-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
corynebacterium glutamcium
IS elements
prophages
genetic switch
“genome healing”
mutagenesis
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2021.751334/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2021.751334/full

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