NON-COENZYME PROPERTIES OF THIAMINE: EVALUATION OF BINDING AFFINITY TO MALATE DEHYDROGENASE ISOFORMS
The aim of this work was evaluation of binding affinity of thiamine to malate dehydrogenase isoforms. The methods of affinity chromatography, SDS PAGE electrophoresis, and MALDI-TOF mass spectroscopy, as well as molecular modeling in silico were used in the study. Affinity sorbent (T-AS) contained C...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-08-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/4_2020/mezhenska_4_2020.pdf |