NON-COENZYME PROPERTIES OF THIAMINE: EVALUATION OF BINDING AFFINITY TO MALATE DEHYDROGENASE ISOFORMS

NON-COENZYME PROPERTIES OF THIAMINE: EVALUATION OF BINDING AFFINITY TO MALATE DEHYDROGENASE ISOFORMS

The aim of this work was evaluation of binding affinity of thiamine to malate dehydrogenase isoforms. The methods of affinity chromatography, SDS PAGE electrophoresis, and MALDI-TOF mass spectroscopy, as well as molecular modeling in silico were used in the study. Affinity sorbent (T-AS) contained C...

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Bibliographic Details
Main Authors:	O. Mezhenska, A. Rebriev, O. Kobzar, N. Zlatoust, A. Vovk, Yu. Parkhomenko
Format:	Article
Language:	English
Published:	National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 2020-08-01
Series:	Biotechnologia Acta
Subjects:	thiamine affinity chromatography malate dehydrogenase protein affinity for thiamine molecular docking thiamine binding protein.
Online Access:	http://biotechnology.kiev.ua/images/storage/4_2020/mezhenska_4_2020.pdf

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