Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli

Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli

Abstract Background Reverse transcription quantitative real-time PCR (RT-qPCR) is a widely used approach for investigating gene expression levels in plants because of its high reproducibility, sensitivity, accuracy and rapidness. Evaluation of reference genes for normalizing RT-qPCR data is a necess...

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Bibliographic Details
Main Authors: Huapeng Sun, Xuefei Jiang, Mengli Sun, Hanqing Cong, Fei Qiao
Format: Article
Language:English
Published: BMC 2019-03-01
Series:Plant Methods
Subjects:
RT-qPCR
Reference gene
C. hainanensis
Expression stability
Normalization
Online Access:http://link.springer.com/article/10.1186/s13007-019-0415-y

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http://link.springer.com/article/10.1186/s13007-019-0415-y

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