Comparison of Single-Stranded DNA Probes Conjugated with Magnetic Particles for Trans-Cleavage in Cas12a-Based Biosensors

Comparison of Single-Stranded DNA Probes Conjugated with Magnetic Particles for Trans-Cleavage in Cas12a-Based Biosensors

Biosensors based on endonuclease Cas12 provide high specificity in pathogen detection. Sensitive detection using Cas12-based assays can be achieved using trans-cleaved DNA probes attached to simply separated carriers, such as magnetic particles (MPs). The aim of this work was to compare polyA, polyC...

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Bibliographic Details
Main Authors: Aleksandr V. Ivanov, Irina V. Safenkova, Anatoly V. Zherdev, Yi Wan, Boris B. Dzantiev
Format: Article
Language:English
Published: MDPI AG 2023-07-01
Series:Biosensors
Subjects:
CRISPR–Cas12
magnetic particles
trans-cleavage
DNA amplification
ssDNA probe
isothermal amplification
Online Access:https://www.mdpi.com/2079-6374/13/7/700

Internet

https://www.mdpi.com/2079-6374/13/7/700

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