SiMeEx, a simplified method for metabolite extraction of adherent mammalian cells

SiMeEx, a simplified method for metabolite extraction of adherent mammalian cells

A reliable method for metabolite extraction is central to mass spectrometry-based metabolomics. However, existing methods are lengthy, mostly due to the step of scraping cells from cell culture vessels, which restricts metabolomics in broader application such as lower cell numbers and high-throughpu...

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Bibliographic Details
Main Authors: Antonia Henne, Anna Vigh, Andre Märtens, Yannic Nonnenmacher, Melanie Ohm, Shirin Hosseini, Tushar H. More, Mario A. Lauterbach, Hendrikus Garritsen, Martin Korte, Wei He, Karsten Hiller
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-12-01
Series:Frontiers in Molecular Biosciences
Subjects:
mammalian cells
GC-MS
metabolite extraction
metabolomics
stable isotope labeling
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2022.1084060/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2022.1084060/full

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