A simplified duplex real-time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures

A simplified duplex real-time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most pathogenic and economically important mycoplasma pathogens that infect chickens. The development of rapid and innovative molecular diagnostic techniques is of pivotal importance for their effective control. The aim of the presen...

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Bibliographic Details
Main Authors: S. Ehtisham-ul-Haque, S.U. Rahman, M.I. Khan, M. Younus, M.M. Awais, A. Nasir
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2015-05-01
Series:Veterinární Medicína
Subjects:
avian mycoplasma spp.
duplex real-time pcr
mgb probe
mgc2 gene
vlha gene
internal positive control
Online Access:https://vetmed.agriculturejournals.cz/artkey/vet-201505-0006_a-simplified-duplex-real-time-pcr-incorporating-taqman-minor-groove-binder-mgb-probes-and-an-exogenous-intern.php

Internet

https://vetmed.agriculturejournals.cz/artkey/vet-201505-0006_a-simplified-duplex-real-time-pcr-incorporating-taqman-minor-groove-binder-mgb-probes-and-an-exogenous-intern.php

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