Genotyping of Single Nucleotide Polymorphisms Using Allele-Specific qPCR Producing Amplicons of Small Sizes Directly from Crude Serum Isolated from Capillary Blood by a Hand-Powered Paper Centrifuge

Genotyping of Single Nucleotide Polymorphisms Using Allele-Specific qPCR Producing Amplicons of Small Sizes Directly from Crude Serum Isolated from Capillary Blood by a Hand-Powered Paper Centrifuge

The cell-free genomic DNA (gDNA) concentration in serum ranges from 1500 to 7500 copies/mL within 2 h after phlebotomy (6–24 times the concentration observed in plasma). Here, we aimed to evaluate the gDNA size distribution in serum with time after coagulation and to test if crude serum ca...

Full description

Bibliographic Details
Main Authors: Gustavo Barcelos Barra, Ticiane Henriques Santa Rita, Daniella Paniago Jardim, Pedro Góes Mesquita, Camila Santos Nobre, Rafael Henriques Jácomo, Lídia Freire Abdalla Nery
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Diagnostics
Subjects:
single nucleotide polymorphism
real-time polymerase chain reaction
serum
Online Access:http://www.mdpi.com/2075-4418/9/1/9

Internet

http://www.mdpi.com/2075-4418/9/1/9

Similar Items