Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition

Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition

ABSTRACT Explorations of complex microbiomes using genomics greatly enhance our understanding about their diversity, biogeography, and function. The isolation of DNA from microbiome specimens is a key prerequisite for such examinations, but challenges remain in obtaining sufficient DNA quantities re...

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Bibliographic Details
Main Authors: Berith E. Knudsen, Lasse Bergmark, Patrick Munk, Oksana Lukjancenko, Anders Priemé, Frank M. Aarestrup, Sünje J. Pamp
Format: Article
Language:English
Published: American Society for Microbiology 2016-10-01
Series:mSystems
Subjects:
16S rRNA gene profiling
DNA isolation
metagenomics
microbial ecology
microbiome
next-generation sequencing
Online Access:https://journals.asm.org/doi/10.1128/mSystems.00095-16

Internet

https://journals.asm.org/doi/10.1128/mSystems.00095-16

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