Challenges of microsatellite development in Lepidoptera: Euphydryas aurinia (Nymphalidae) as a case study

Currently it remains difficult to obtain robust microsatellite markers for Lepidoptera. In an attempt to overcome the problems associated with developing microsatellite markers for this insect order we combined (i) biotin-enrichment protocol, (ii) next generation pyrosequencing (through 454 GS-FLX T...

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Bibliographic Details
Main Authors: Melthide SINAMA, Vincent DUBUT, Caroline COSTEDOAT, André GILLES, Marius JUNKER, Thibaut MALAUSA, MARTIN Jean-François, Gabriel NÈVE, Nicolas PECH, Thomas SCHMITT, Marie ZIMMERMANN, Emese MEGLÉCZ
Format: Article
Language:English
Published: Institute of Entomology, Biology Centre, Czech Academy of Science 2011-04-01
Series:European Journal of Entomology
Subjects:
Online Access:https://www.eje.cz/artkey/eje-201102-0013_Challenges_of_microsatellite_development_in_Lepidoptera_Euphydryas_aurinia_Nymphalidae_as_a_case_study.php