Extraction of Total RNA from Leaves of Eucalyptus and Other Woody and Herbaceous Plants Using Sodium Isoascorbate
Rapid extraction of total RNA from Eucalyptus leaves is difficult due to the high content of polyphenolics and polysaccharides. A rapid and simple method was developed by using an extraction buffer containing sodium isoascorbate at a concentration of 500 mM. This method consisted of one or two chlor...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03345st05 |