Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study
The aim of the study was to develop a sustained-release varnish (SRV) containing chlorhexidine (CHX) for sinonasal stents (SNS) to reduce bacterial growth and biofilm formation in the sinonasal cavity. Segments of SNS were coated with SRV-CHX or SRV-placebo and exposed daily to bacterial cultures of...
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MDPI AG
2021-10-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/11/1783 |
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| author | Alessandra Cataldo Russomando Ronit Vogt Sionov Michael Friedman Irith Gati Ron Eliashar Doron Steinberg Menachem Gross |
| author_facet | Alessandra Cataldo Russomando Ronit Vogt Sionov Michael Friedman Irith Gati Ron Eliashar Doron Steinberg Menachem Gross |
| author_sort | Alessandra Cataldo Russomando |
| collection | DOAJ |
| description | The aim of the study was to develop a sustained-release varnish (SRV) containing chlorhexidine (CHX) for sinonasal stents (SNS) to reduce bacterial growth and biofilm formation in the sinonasal cavity. Segments of SNS were coated with SRV-CHX or SRV-placebo and exposed daily to bacterial cultures of <i>Staphylococcus aureus subsp. aureus</i> ATCC 25923 or <i>Pseudomonas aeruginosa</i> ATCC HER-1018 (PAO1). Anti-bacterial effects were assessed by disc diffusion assay and planktonic-based activity assay. Biofilm formation on the coated stents was visualized by confocal laser scanning microscopy (CLSM) and high-resolution scanning electron microscopy (HR-SEM). The metabolic activity of the biofilms was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method. Disc diffusion assay showed that SRV-CHX-coated SNS segments inhibited bacterial growth of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 for 26 days and <i>P. aeruginosa</i> ATCC HER-1018 for 19 days. CHX was released from coated SNS segments in a pH 6 medium up to 30 days, resulting in growth inhibition of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 for 22 days and <i>P. aeruginosa</i> ATCC HER-1018 for 24 days. The MTT assay showed a reduction of biofilm growth on the coated SNS by 69% for <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 and 40% for <i>P. aeruginosa</i> ATCC HER-1018 compared to the placebo stent after repeated exposure to planktonic growing bacteria. CLSM and HR-SEM showed a significant reduction of biofilm formation on the SRV-CHX-coated SNS segments. Coating of SNS with SRV-CHX maintains a sustained delivery of CHX, providing an inhibitory effect on the bacterial growth of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 and <i>P. aeruginosa</i> ATCC HER-1018 for approximately 3 weeks. |
| first_indexed | 2024-03-10T05:08:42Z |
| format | Article |
| id | doaj.art-275d82ae5b854ee9a0d12f2e0f99aeee |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T05:08:42Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-275d82ae5b854ee9a0d12f2e0f99aeee2023-11-23T00:57:33ZengMDPI AGPharmaceutics1999-49232021-10-011311178310.3390/pharmaceutics13111783Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro StudyAlessandra Cataldo Russomando0Ronit Vogt Sionov1Michael Friedman2Irith Gati3Ron Eliashar4Doron Steinberg5Menachem Gross6Department of Otolaryngology-Head and Neck Surgery, Hadassah Medical Center, Jerusalem 9112102, IsraelThe Biofilm Research Laboratory, The Institute of Dental Sciences, The Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelInstitute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelInstitute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelDepartment of Otolaryngology-Head and Neck Surgery, Hadassah Medical Center, Jerusalem 9112102, IsraelThe Biofilm Research Laboratory, The Institute of Dental Sciences, The Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelDepartment of Otolaryngology-Head and Neck Surgery, Hadassah Medical Center, Jerusalem 9112102, IsraelThe aim of the study was to develop a sustained-release varnish (SRV) containing chlorhexidine (CHX) for sinonasal stents (SNS) to reduce bacterial growth and biofilm formation in the sinonasal cavity. Segments of SNS were coated with SRV-CHX or SRV-placebo and exposed daily to bacterial cultures of <i>Staphylococcus aureus subsp. aureus</i> ATCC 25923 or <i>Pseudomonas aeruginosa</i> ATCC HER-1018 (PAO1). Anti-bacterial effects were assessed by disc diffusion assay and planktonic-based activity assay. Biofilm formation on the coated stents was visualized by confocal laser scanning microscopy (CLSM) and high-resolution scanning electron microscopy (HR-SEM). The metabolic activity of the biofilms was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method. Disc diffusion assay showed that SRV-CHX-coated SNS segments inhibited bacterial growth of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 for 26 days and <i>P. aeruginosa</i> ATCC HER-1018 for 19 days. CHX was released from coated SNS segments in a pH 6 medium up to 30 days, resulting in growth inhibition of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 for 22 days and <i>P. aeruginosa</i> ATCC HER-1018 for 24 days. The MTT assay showed a reduction of biofilm growth on the coated SNS by 69% for <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 and 40% for <i>P. aeruginosa</i> ATCC HER-1018 compared to the placebo stent after repeated exposure to planktonic growing bacteria. CLSM and HR-SEM showed a significant reduction of biofilm formation on the SRV-CHX-coated SNS segments. Coating of SNS with SRV-CHX maintains a sustained delivery of CHX, providing an inhibitory effect on the bacterial growth of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 and <i>P. aeruginosa</i> ATCC HER-1018 for approximately 3 weeks.https://www.mdpi.com/1999-4923/13/11/1783bacteria biofilmbiofilm inhibitionchlorhexidinechronic rhinosinusitisnasal stent<i>Pseudomonas aeruginosa</i> |
| spellingShingle | Alessandra Cataldo Russomando Ronit Vogt Sionov Michael Friedman Irith Gati Ron Eliashar Doron Steinberg Menachem Gross Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study Pharmaceutics bacteria biofilm biofilm inhibition chlorhexidine chronic rhinosinusitis nasal stent <i>Pseudomonas aeruginosa</i> |
| title | Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study |
| title_full | Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study |
| title_fullStr | Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study |
| title_full_unstemmed | Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study |
| title_short | Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study |
| title_sort | sinonasal stent coated with slow release varnish of chlorhexidine has sustained protection against bacterial biofilm growth in the sinonasal cavity an in vitro study |
| topic | bacteria biofilm biofilm inhibition chlorhexidine chronic rhinosinusitis nasal stent <i>Pseudomonas aeruginosa</i> |
| url | https://www.mdpi.com/1999-4923/13/11/1783 |
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