Mapping Transgene Insertion Sites Reveals Complex Interactions Between Mouse Transgenes and Neighboring Endogenous Genes

Mapping Transgene Insertion Sites Reveals Complex Interactions Between Mouse Transgenes and Neighboring Endogenous Genes

Transgenic mouse lines are routinely employed to label and manipulate distinct cell types. The transgene generally comprises cell-type specific regulatory elements linked to a cDNA encoding a reporter or other protein. However, off-target expression seemingly unrelated to the regulatory elements in...

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Bibliographic Details
Main Authors: Mallory A. Laboulaye, Xin Duan, Mu Qiao, Irene E. Whitney, Joshua R. Sanes
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-10-01
Series:Frontiers in Molecular Neuroscience
Subjects:
transgenic mice
GFP
Mnx1
Khdrbs2
Fat4
retina
Online Access:https://www.frontiersin.org/article/10.3389/fnmol.2018.00385/full

Internet

https://www.frontiersin.org/article/10.3389/fnmol.2018.00385/full

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