Optimization of Isolation and Transformation of Protoplasts from <i>Uncaria rhynchophylla</i> and Its Application to Transient Gene Expression Analysis

Optimization of Isolation and Transformation of Protoplasts from <i>Uncaria rhynchophylla</i> and Its Application to Transient Gene Expression Analysis

Protoplast-based engineering has become an important tool for basic plant molecular biology research and developing genome-edited crops. <i>Uncaria rhynchophylla</i> is a traditional Chinese medicinal plant with a variety of pharmaceutically important indole alkaloids. In this study, an...

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Bibliographic Details
Main Authors: Yingying Shao, Detian Mu, Limei Pan, Iain W. Wilson, Yajie Zheng, Lina Zhu, Zhiguo Lu, Lingyun Wan, Jine Fu, Shugen Wei, Lisha Song, Deyou Qiu, Qi Tang
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Uncaria rhynchophylla</i>
protoplast isolation
PEG-mediated transfection
subcellular localization
dual-luciferase (Dual-LUC) assays
Online Access:https://www.mdpi.com/1422-0067/24/4/3633

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https://www.mdpi.com/1422-0067/24/4/3633

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