Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4+ T Cells From HIV-1 Patients

Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4+ T Cells From HIV-1 Patients

We described a novel HIV autologous isolation method based in coculturing macrophages and CD4+T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 1010HIV RNA copies...

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Bibliographic Details
Main Authors: Cristina Xufré, Tanía González, Lorna Leal, Charles M. Trubey, Jeffrey D. Lifson, José María Gatell, José Alcamí, Núria Climent, Felipe García, Sonsoles Sánchez-Palomino
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-04-01
Series:Frontiers in Virology
Subjects:
autologous HIV immunogen
HIV isolation
HIV therapeutic vaccine
CD4+ T cells
coculture
monocyte-derived macrophages (MDM)
Online Access:https://www.frontiersin.org/articles/10.3389/fviro.2022.869431/full

Internet

https://www.frontiersin.org/articles/10.3389/fviro.2022.869431/full

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