Coupling live-cell imaging and in situ isolation of the same single cell to profile the transient states of predicted drug-tolerant cells

Coupling live-cell imaging and in situ isolation of the same single cell to profile the transient states of predicted drug-tolerant cells

Summary: Cell response variability is a starting point in cancer drug resistance that has been difficult to analyze because the tolerant cell states are short lived. Here, we present fate-seq, an approach to isolate single cells in their transient states of drug sensitivity or tolerance before profi...

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Bibliographic Details
Main Authors: Benjamin Bian, Agnès Paquet, Marie-Jeanne Arguel, Mickael Meyer, Ludovic Peyre, Asma Chalabi, Marielle Péré, Kevin Lebrigand, Rainer Waldmann, Pascal Barbry, Paul Hofman, Jérémie Roux
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Cell-based Assays
Microscopy
Systems biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004804

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http://www.sciencedirect.com/science/article/pii/S2666166722004804

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