Structural Characteristics of <i>Rehmannia glutinosa</i> Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells
Polysaccharide decolorization is a key determinant of polysaccharide structure. In this study, two purified <i>Rehmannia glutinosa</i> polysaccharides, RGP−1−A and RGP−2−A, were obtained after decolorization using the AB-8 macroporous resin and H<sub>2</sub>O<sub>2</...
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2022-10-01
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| author | Heng Ren Zhongyuan Li Rui Gao Tongxi Zhao Dan Luo Zihao Yu Shuang Zhang Chen Qi Yaqi Wang Hanzhen Qiao Yaoming Cui Liping Gan Peng Wang Jinrong Wang |
| author_facet | Heng Ren Zhongyuan Li Rui Gao Tongxi Zhao Dan Luo Zihao Yu Shuang Zhang Chen Qi Yaqi Wang Hanzhen Qiao Yaoming Cui Liping Gan Peng Wang Jinrong Wang |
| author_sort | Heng Ren |
| collection | DOAJ |
| description | Polysaccharide decolorization is a key determinant of polysaccharide structure. In this study, two purified <i>Rehmannia glutinosa</i> polysaccharides, RGP−1−A and RGP−2−A, were obtained after decolorization using the AB-8 macroporous resin and H<sub>2</sub>O<sub>2</sub>, respectively. RGP−1−A (molecular weight (Mw) = 18,964 Da) and RGP−2−A (Mw = 3305 Da) were acidic and neutral heteropolysaccharides, respectively, and were both polycrystalline in structure. FTIR analysis revealed that RGP−1−A was a sulfate polysaccharide, while RGP−2−A had no sulfate group. Experiments on IPEC-1 cells showed that RGPs alleviated oxidative stress by regulating the Nrf2/Keap1 pathway. These findings were confirmed by the upregulation of Nrf2, NQO1, and HO-1; the subsequent increase in the levels of antioxidant indicators (SOD, LDH, CAT, and MDA); and the restoration of mitochondrial membrane potential. Overall, the antioxidant capacity of RGP−1−A was significantly higher than that of RGP−2−A. These results suggest that RGPs may be a potential natural antioxidant and could be developed into functional foods. |
| first_indexed | 2024-03-09T19:04:16Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T19:04:16Z |
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| series | Foods |
| spelling | doaj.art-27de9baab06c4a93b0b2dc59845ef64b2023-11-24T04:41:01ZengMDPI AGFoods2304-81582022-10-011121344910.3390/foods11213449Structural Characteristics of <i>Rehmannia glutinosa</i> Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial CellsHeng Ren0Zhongyuan Li1Rui Gao2Tongxi Zhao3Dan Luo4Zihao Yu5Shuang Zhang6Chen Qi7Yaqi Wang8Hanzhen Qiao9Yaoming Cui10Liping Gan11Peng Wang12Jinrong Wang13College of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biology Engineering, Henan University of Technology, Zhengzhou 450001, ChinaPolysaccharide decolorization is a key determinant of polysaccharide structure. In this study, two purified <i>Rehmannia glutinosa</i> polysaccharides, RGP−1−A and RGP−2−A, were obtained after decolorization using the AB-8 macroporous resin and H<sub>2</sub>O<sub>2</sub>, respectively. RGP−1−A (molecular weight (Mw) = 18,964 Da) and RGP−2−A (Mw = 3305 Da) were acidic and neutral heteropolysaccharides, respectively, and were both polycrystalline in structure. FTIR analysis revealed that RGP−1−A was a sulfate polysaccharide, while RGP−2−A had no sulfate group. Experiments on IPEC-1 cells showed that RGPs alleviated oxidative stress by regulating the Nrf2/Keap1 pathway. These findings were confirmed by the upregulation of Nrf2, NQO1, and HO-1; the subsequent increase in the levels of antioxidant indicators (SOD, LDH, CAT, and MDA); and the restoration of mitochondrial membrane potential. Overall, the antioxidant capacity of RGP−1−A was significantly higher than that of RGP−2−A. These results suggest that RGPs may be a potential natural antioxidant and could be developed into functional foods.https://www.mdpi.com/2304-8158/11/21/3449<i>Rehmannia glutinosa</i>polysaccharidesstructureantioxidant activity |
| spellingShingle | Heng Ren Zhongyuan Li Rui Gao Tongxi Zhao Dan Luo Zihao Yu Shuang Zhang Chen Qi Yaqi Wang Hanzhen Qiao Yaoming Cui Liping Gan Peng Wang Jinrong Wang Structural Characteristics of <i>Rehmannia glutinosa</i> Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells Foods <i>Rehmannia glutinosa</i> polysaccharides structure antioxidant activity |
| title | Structural Characteristics of <i>Rehmannia glutinosa</i> Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells |
| title_full | Structural Characteristics of <i>Rehmannia glutinosa</i> Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells |
| title_fullStr | Structural Characteristics of <i>Rehmannia glutinosa</i> Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells |
| title_full_unstemmed | Structural Characteristics of <i>Rehmannia glutinosa</i> Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells |
| title_short | Structural Characteristics of <i>Rehmannia glutinosa</i> Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells |
| title_sort | structural characteristics of i rehmannia glutinosa i polysaccharides treated using different decolorization processes and their antioxidant effects in intestinal epithelial cells |
| topic | <i>Rehmannia glutinosa</i> polysaccharides structure antioxidant activity |
| url | https://www.mdpi.com/2304-8158/11/21/3449 |
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