Preliminary studies on ribonucleases from Poa pratensis seeds

Ribonuclease was extracted from Poa pratensis seeds with 0.1 M acetate buffer, pH 5.1, and then precipitated with alcohol. The enzyme was separated into 5 fractions (I-V) after chromatography on DEAE-cellulose at pH 5.1. The enzymes were stable at 50°C, at pH 7.1. The activity of ribonucleases I, II...

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Bibliographic Details
Main Authors: Irena Lorenc-Kubis, Bronisława Morawiecka
Format: Article
Language:English
Published: Polish Botanical Society 2015-01-01
Series:Acta Societatis Botanicorum Poloniae
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/5103