Preliminary studies on ribonucleases from Poa pratensis seeds
Ribonuclease was extracted from Poa pratensis seeds with 0.1 M acetate buffer, pH 5.1, and then precipitated with alcohol. The enzyme was separated into 5 fractions (I-V) after chromatography on DEAE-cellulose at pH 5.1. The enzymes were stable at 50°C, at pH 7.1. The activity of ribonucleases I, II...
Main Authors: | Irena Lorenc-Kubis, Bronisława Morawiecka |
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Format: | Article |
Language: | English |
Published: |
Polish Botanical Society
2015-01-01
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Series: | Acta Societatis Botanicorum Poloniae |
Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/5103 |
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