Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions.

The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA. To examine the most ac...

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Bibliographic Details
Main Authors: Yuki Nakayama, Hiromi Yamaguchi, Naoki Einaga, Mariko Esumi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4777359?pdf=render