The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity.
BACKGROUND: Breast cancer is a remarkably heterogeneous disease. Luminal, basal-like, "normal-like", and ERBB2+ subgroups were identified and were shown to have different prognoses. The mechanisms underlying this heterogeneity are poorly understood. In our study, we explored the role of ce...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2891998?pdf=render |
| _version_ | 1828381194897588224 |
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| author | Mine Mumcuoglu Sevgi Bagislar Haluk Yuzugullu Hani Alotaibi Serif Senturk Pelin Telkoparan Bala Gur-Dedeoglu Burcu Cingoz Betul Bozkurt Uygar H Tazebay Isik G Yulug K Can Akcali Mehmet Ozturk |
| author_facet | Mine Mumcuoglu Sevgi Bagislar Haluk Yuzugullu Hani Alotaibi Serif Senturk Pelin Telkoparan Bala Gur-Dedeoglu Burcu Cingoz Betul Bozkurt Uygar H Tazebay Isik G Yulug K Can Akcali Mehmet Ozturk |
| author_sort | Mine Mumcuoglu |
| collection | DOAJ |
| description | BACKGROUND: Breast cancer is a remarkably heterogeneous disease. Luminal, basal-like, "normal-like", and ERBB2+ subgroups were identified and were shown to have different prognoses. The mechanisms underlying this heterogeneity are poorly understood. In our study, we explored the role of cellular differentiation and senescence as a potential cause of heterogeneity. METHODOLOGY/PRINCIPAL FINDINGS: A panel of breast cancer cell lines, isogenic clones, and breast tumors were used. Based on their ability to generate senescent progeny under low-density clonogenic conditions, we classified breast cancer cell lines as senescent cell progenitor (SCP) and immortal cell progenitor (ICP) subtypes. All SCP cell lines expressed estrogen receptor (ER). Loss of ER expression combined with the accumulation of p21(Cip1) correlated with senescence in these cell lines. p21(Cip1) knockdown, estrogen-mediated ER activation or ectopic ER overexpression protected cells against senescence. In contrast, tamoxifen triggered a robust senescence response. As ER expression has been linked to luminal differentiation, we compared the differentiation status of SCP and ICP cell lines using stem/progenitor, luminal, and myoepithelial markers. The SCP cells produced CD24+ or ER+ luminal-like and ASMA+ myoepithelial-like progeny, in addition to CD44+ stem/progenitor-like cells. In contrast, ICP cell lines acted as differentiation-defective stem/progenitor cells. Some ICP cell lines generated only CD44+/CD24-/ER-/ASMA- progenitor/stem-like cells, and others also produced CD24+/ER- luminal-like, but not ASMA+ myoepithelial-like cells. Furthermore, gene expression profiles clustered SCP cell lines with luminal A and "normal-like" tumors, and ICP cell lines with luminal B and basal-like tumors. The ICP cells displayed higher tumorigenicity in immunodeficient mice. CONCLUSIONS/SIGNIFICANCE: Luminal A and "normal-like" breast cancer cell lines were able to generate luminal-like and myoepithelial-like progeny undergoing senescence arrest. In contrast, luminal B/basal-like cell lines acted as stem/progenitor cells with defective differentiation capacities. Our findings suggest that the malignancy of breast tumors is directly correlated with stem/progenitor phenotypes and poor differentiation potential. |
| first_indexed | 2024-12-10T04:11:17Z |
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| id | doaj.art-2834daefa7c942d09e8c08fae1d66d1e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T04:11:17Z |
| publishDate | 2010-01-01 |
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| spelling | doaj.art-2834daefa7c942d09e8c08fae1d66d1e2022-12-22T02:02:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0156e1128810.1371/journal.pone.0011288The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity.Mine MumcuogluSevgi BagislarHaluk YuzugulluHani AlotaibiSerif SenturkPelin TelkoparanBala Gur-DedeogluBurcu CingozBetul BozkurtUygar H TazebayIsik G YulugK Can AkcaliMehmet OzturkBACKGROUND: Breast cancer is a remarkably heterogeneous disease. Luminal, basal-like, "normal-like", and ERBB2+ subgroups were identified and were shown to have different prognoses. The mechanisms underlying this heterogeneity are poorly understood. In our study, we explored the role of cellular differentiation and senescence as a potential cause of heterogeneity. METHODOLOGY/PRINCIPAL FINDINGS: A panel of breast cancer cell lines, isogenic clones, and breast tumors were used. Based on their ability to generate senescent progeny under low-density clonogenic conditions, we classified breast cancer cell lines as senescent cell progenitor (SCP) and immortal cell progenitor (ICP) subtypes. All SCP cell lines expressed estrogen receptor (ER). Loss of ER expression combined with the accumulation of p21(Cip1) correlated with senescence in these cell lines. p21(Cip1) knockdown, estrogen-mediated ER activation or ectopic ER overexpression protected cells against senescence. In contrast, tamoxifen triggered a robust senescence response. As ER expression has been linked to luminal differentiation, we compared the differentiation status of SCP and ICP cell lines using stem/progenitor, luminal, and myoepithelial markers. The SCP cells produced CD24+ or ER+ luminal-like and ASMA+ myoepithelial-like progeny, in addition to CD44+ stem/progenitor-like cells. In contrast, ICP cell lines acted as differentiation-defective stem/progenitor cells. Some ICP cell lines generated only CD44+/CD24-/ER-/ASMA- progenitor/stem-like cells, and others also produced CD24+/ER- luminal-like, but not ASMA+ myoepithelial-like cells. Furthermore, gene expression profiles clustered SCP cell lines with luminal A and "normal-like" tumors, and ICP cell lines with luminal B and basal-like tumors. The ICP cells displayed higher tumorigenicity in immunodeficient mice. CONCLUSIONS/SIGNIFICANCE: Luminal A and "normal-like" breast cancer cell lines were able to generate luminal-like and myoepithelial-like progeny undergoing senescence arrest. In contrast, luminal B/basal-like cell lines acted as stem/progenitor cells with defective differentiation capacities. Our findings suggest that the malignancy of breast tumors is directly correlated with stem/progenitor phenotypes and poor differentiation potential.http://europepmc.org/articles/PMC2891998?pdf=render |
| spellingShingle | Mine Mumcuoglu Sevgi Bagislar Haluk Yuzugullu Hani Alotaibi Serif Senturk Pelin Telkoparan Bala Gur-Dedeoglu Burcu Cingoz Betul Bozkurt Uygar H Tazebay Isik G Yulug K Can Akcali Mehmet Ozturk The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity. PLoS ONE |
| title | The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity. |
| title_full | The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity. |
| title_fullStr | The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity. |
| title_full_unstemmed | The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity. |
| title_short | The ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity. |
| title_sort | ability to generate senescent progeny as a mechanism underlying breast cancer cell heterogeneity |
| url | http://europepmc.org/articles/PMC2891998?pdf=render |
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