Expanded targeting scope of LbCas12a variants allows editing of multiple oncogenic mutations

Expanded targeting scope of LbCas12a variants allows editing of multiple oncogenic mutations

RNA-guided CRISPR-Cas12a endonucleases are promising tools for genome engineering. Here we demonstrate that LbCas12a variants derived from Lachnospiraceae bacterium show a broad PAM preference, recognizing certain non-canonical PAMs with high efficiency. Furthermore, we engineered LbABE8e to carry G...

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Bibliographic Details
Main Authors: Eunyoung Choi, Hye-Yeon Hwang, Eunji Kwon, Daesik Kim, Taeyoung Koo
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: RNA/DNA editing
LbCas12a
LbABE8e
CRISPR
non-canonical PAMs
colorectal cancer
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253122002487

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http://www.sciencedirect.com/science/article/pii/S2162253122002487

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