A high-resolution view of genome-wide pneumococcal transformation.
Transformation is an important mechanism of microbial evolution through which bacteria have been observed to rapidly adapt in response to clinical interventions; examples include facilitating vaccine evasion and the development of penicillin resistance in the major respiratory pathogen Streptococcus...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC3375284?pdf=render |
| _version_ | 1819074050513698816 |
|---|---|
| author | Nicholas J Croucher Simon R Harris Lars Barquist Julian Parkhill Stephen D Bentley |
| author_facet | Nicholas J Croucher Simon R Harris Lars Barquist Julian Parkhill Stephen D Bentley |
| author_sort | Nicholas J Croucher |
| collection | DOAJ |
| description | Transformation is an important mechanism of microbial evolution through which bacteria have been observed to rapidly adapt in response to clinical interventions; examples include facilitating vaccine evasion and the development of penicillin resistance in the major respiratory pathogen Streptococcus pneumoniae. To characterise the process in detail, the genomes of 124 S. pneumoniae isolates produced through in vitro transformation were sequenced and recombination events detected. Those recombinations importing the selected marker were independent of unselected events elsewhere in the genome, the positions of which were not significantly affected by local sequence similarity between donor and recipient or mismatch repair processes. However, both types of recombinations were sometimes mosaic, with multiple non-contiguous segments originating from the same molecule of donor DNA. The lengths of the unselected events were exponentially distributed with a mean of 2.3 kb, implying that recombinations are stochastically resolved with a fixed per base probability of 4.4×10(-4) bp(-1). This distribution of recombination sizes, coupled with an observed under representation of large insertions within transferred sequence, suggests transformation has the potential to reduce the size of bacterial genomes, and is unlikely to act as an efficient mechanism for the uptake of accessory genomic loci. |
| first_indexed | 2024-12-21T18:03:21Z |
| format | Article |
| id | doaj.art-284d9916ccd6420c9bb6251517ef879b |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-21T18:03:21Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-284d9916ccd6420c9bb6251517ef879b2022-12-21T18:55:01ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742012-01-0186e100274510.1371/journal.ppat.1002745A high-resolution view of genome-wide pneumococcal transformation.Nicholas J CroucherSimon R HarrisLars BarquistJulian ParkhillStephen D BentleyTransformation is an important mechanism of microbial evolution through which bacteria have been observed to rapidly adapt in response to clinical interventions; examples include facilitating vaccine evasion and the development of penicillin resistance in the major respiratory pathogen Streptococcus pneumoniae. To characterise the process in detail, the genomes of 124 S. pneumoniae isolates produced through in vitro transformation were sequenced and recombination events detected. Those recombinations importing the selected marker were independent of unselected events elsewhere in the genome, the positions of which were not significantly affected by local sequence similarity between donor and recipient or mismatch repair processes. However, both types of recombinations were sometimes mosaic, with multiple non-contiguous segments originating from the same molecule of donor DNA. The lengths of the unselected events were exponentially distributed with a mean of 2.3 kb, implying that recombinations are stochastically resolved with a fixed per base probability of 4.4×10(-4) bp(-1). This distribution of recombination sizes, coupled with an observed under representation of large insertions within transferred sequence, suggests transformation has the potential to reduce the size of bacterial genomes, and is unlikely to act as an efficient mechanism for the uptake of accessory genomic loci.http://europepmc.org/articles/PMC3375284?pdf=render |
| spellingShingle | Nicholas J Croucher Simon R Harris Lars Barquist Julian Parkhill Stephen D Bentley A high-resolution view of genome-wide pneumococcal transformation. PLoS Pathogens |
| title | A high-resolution view of genome-wide pneumococcal transformation. |
| title_full | A high-resolution view of genome-wide pneumococcal transformation. |
| title_fullStr | A high-resolution view of genome-wide pneumococcal transformation. |
| title_full_unstemmed | A high-resolution view of genome-wide pneumococcal transformation. |
| title_short | A high-resolution view of genome-wide pneumococcal transformation. |
| title_sort | high resolution view of genome wide pneumococcal transformation |
| url | http://europepmc.org/articles/PMC3375284?pdf=render |
| work_keys_str_mv | AT nicholasjcroucher ahighresolutionviewofgenomewidepneumococcaltransformation AT simonrharris ahighresolutionviewofgenomewidepneumococcaltransformation AT larsbarquist ahighresolutionviewofgenomewidepneumococcaltransformation AT julianparkhill ahighresolutionviewofgenomewidepneumococcaltransformation AT stephendbentley ahighresolutionviewofgenomewidepneumococcaltransformation AT nicholasjcroucher highresolutionviewofgenomewidepneumococcaltransformation AT simonrharris highresolutionviewofgenomewidepneumococcaltransformation AT larsbarquist highresolutionviewofgenomewidepneumococcaltransformation AT julianparkhill highresolutionviewofgenomewidepneumococcaltransformation AT stephendbentley highresolutionviewofgenomewidepneumococcaltransformation |