Rapamycin prevents retinal neovascularization by downregulation of cyclin D1 in a mouse model of oxygen-induced retinopathy
Abstract Background Rapamycin (RAPA) is a potent angiogenic inhibitor and the aim of this study is to identify the inhibitory effect of RAPA on retinal neovascularization (RNV) in experimental oxygen-induced retinopathy (OIR). Methods Forty-two 7-day-old C57BL/6 J mice were randomly divided into nor...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-02-01
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| Series: | BMC Ophthalmology |
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| Online Access: | https://doi.org/10.1186/s12886-020-1325-5 |
| _version_ | 1818676748312641536 |
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| author | Feng Jiang Ying Wang Shufang Du Heng Jin Jindong Han |
| author_facet | Feng Jiang Ying Wang Shufang Du Heng Jin Jindong Han |
| author_sort | Feng Jiang |
| collection | DOAJ |
| description | Abstract Background Rapamycin (RAPA) is a potent angiogenic inhibitor and the aim of this study is to identify the inhibitory effect of RAPA on retinal neovascularization (RNV) in experimental oxygen-induced retinopathy (OIR). Methods Forty-two 7-day-old C57BL/6 J mice were randomly divided into normoxia control group (14 mice), OIR group (14 mice), and rapamycin (RAPA) group. OIR model was induced in OIR and RAPA group. Vehicle and RAPA (2 mg/kg/d) was injected intraperitoneally daily from postnatal day 12 (P12) in OIR and RAPA groups, respectively. RNV was evaluated using fluorescence angiography and histopathology on P17. Non-perfused areas of retina were analyzed by Image-Pro plus 6.0 software. Retinal expression of cyclin D1 was detected both at mRNA and protein levels. Results RAPA treatment significantly decreased RNV, non-perfused areas and number of endothelial cell nuclei breaking through the internal limiting membrane (ILM) in OIR mice. Moreover, RAPA decreased activation of cyclin D1 in retina caused by OIR. Conclusion RAPA can inhibit RNV by downregulating the expression of cyclin D1, which indicates its therapeutic potential in treating RNV-related diseases. |
| first_indexed | 2024-12-17T08:48:24Z |
| format | Article |
| id | doaj.art-2851a9d4d3124733ac74466a00d35486 |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-12-17T08:48:24Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-2851a9d4d3124733ac74466a00d354862022-12-21T21:56:09ZengBMCBMC Ophthalmology1471-24152020-02-012011510.1186/s12886-020-1325-5Rapamycin prevents retinal neovascularization by downregulation of cyclin D1 in a mouse model of oxygen-induced retinopathyFeng Jiang0Ying Wang1Shufang Du2Heng Jin3Jindong Han4Department of Ophthalmology, Tianjin Medical University General HospitalDepartment of Ophthalmology, Shenyang Aier Eye HospitalDepartment of Ophthalmology, Shanxi Eye HospitalDepartment of Emergency Medicine, Tianjin Medical University General HospitalDepartment of Vitreous and Retina, Tianjin Medical University Eye HospitalAbstract Background Rapamycin (RAPA) is a potent angiogenic inhibitor and the aim of this study is to identify the inhibitory effect of RAPA on retinal neovascularization (RNV) in experimental oxygen-induced retinopathy (OIR). Methods Forty-two 7-day-old C57BL/6 J mice were randomly divided into normoxia control group (14 mice), OIR group (14 mice), and rapamycin (RAPA) group. OIR model was induced in OIR and RAPA group. Vehicle and RAPA (2 mg/kg/d) was injected intraperitoneally daily from postnatal day 12 (P12) in OIR and RAPA groups, respectively. RNV was evaluated using fluorescence angiography and histopathology on P17. Non-perfused areas of retina were analyzed by Image-Pro plus 6.0 software. Retinal expression of cyclin D1 was detected both at mRNA and protein levels. Results RAPA treatment significantly decreased RNV, non-perfused areas and number of endothelial cell nuclei breaking through the internal limiting membrane (ILM) in OIR mice. Moreover, RAPA decreased activation of cyclin D1 in retina caused by OIR. Conclusion RAPA can inhibit RNV by downregulating the expression of cyclin D1, which indicates its therapeutic potential in treating RNV-related diseases.https://doi.org/10.1186/s12886-020-1325-5Retinal neovascularizationPreventionRapamycinCell cycleAnimal experiment |
| spellingShingle | Feng Jiang Ying Wang Shufang Du Heng Jin Jindong Han Rapamycin prevents retinal neovascularization by downregulation of cyclin D1 in a mouse model of oxygen-induced retinopathy BMC Ophthalmology Retinal neovascularization Prevention Rapamycin Cell cycle Animal experiment |
| title | Rapamycin prevents retinal neovascularization by downregulation of cyclin D1 in a mouse model of oxygen-induced retinopathy |
| title_full | Rapamycin prevents retinal neovascularization by downregulation of cyclin D1 in a mouse model of oxygen-induced retinopathy |
| title_fullStr | Rapamycin prevents retinal neovascularization by downregulation of cyclin D1 in a mouse model of oxygen-induced retinopathy |
| title_full_unstemmed | Rapamycin prevents retinal neovascularization by downregulation of cyclin D1 in a mouse model of oxygen-induced retinopathy |
| title_short | Rapamycin prevents retinal neovascularization by downregulation of cyclin D1 in a mouse model of oxygen-induced retinopathy |
| title_sort | rapamycin prevents retinal neovascularization by downregulation of cyclin d1 in a mouse model of oxygen induced retinopathy |
| topic | Retinal neovascularization Prevention Rapamycin Cell cycle Animal experiment |
| url | https://doi.org/10.1186/s12886-020-1325-5 |
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