Hydrothermal extraction, structural characterization, and inhibition HeLa cells proliferation of functional polysaccharides from Chinese tea Zhongcha 108
Five subfractions of functional polysaccharides were extracted by hydrothermal technology from Chinese tea Zhongcha 108, followed by purification using DEAE-52 column chromatography. Their structural characteristics were investigated by sugar analysis, GPC, FT-IR, AFM, methylation analysis, and 2D H...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617305819 |
| _version_ | 1818952028372598784 |
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| author | Yuefang Gao Yubo Zhou Qiang Zhang Kun Zhang Pai Peng Liangchao Chen Bin Xiao |
| author_facet | Yuefang Gao Yubo Zhou Qiang Zhang Kun Zhang Pai Peng Liangchao Chen Bin Xiao |
| author_sort | Yuefang Gao |
| collection | DOAJ |
| description | Five subfractions of functional polysaccharides were extracted by hydrothermal technology from Chinese tea Zhongcha 108, followed by purification using DEAE-52 column chromatography. Their structural characteristics were investigated by sugar analysis, GPC, FT-IR, AFM, methylation analysis, and 2D HSQC NMR spectrum. The results showed that the tea functional polysaccharides were mainly consisted of acidic polysaccharide rich in glucuronic acid and neutral polysaccharides composition of rhamnose, galactose, and glucose. They had a backbone of 1,4-linked α-d-Glcp and 1,2,4-linked α-l-Rhap with side chains of 1- and 1,6-linked α-d-Galp substituted at C-2 of α-l-Rhap. Furthermore, anti-cancer activities of the polysaccharides were evaluated by inhibition assays of HeLa cells proliferation. Interestingly, it was found that the acidic polysaccharide showed markedly increased effect while neutral polysaccharides F0.1-F0.3 exhibited markedly inhibitory effects on the proliferation of HeLa cells, the highest inhibition rate can reach up to 10.43% treated by F0.3 at 400 μg/ml for 48 h. |
| first_indexed | 2024-12-20T09:43:52Z |
| format | Article |
| id | doaj.art-2871b26f30724b438fff896ef27ac21e |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T09:43:52Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-2871b26f30724b438fff896ef27ac21e2022-12-21T19:44:48ZengElsevierJournal of Functional Foods1756-46462017-12-013918Hydrothermal extraction, structural characterization, and inhibition HeLa cells proliferation of functional polysaccharides from Chinese tea Zhongcha 108Yuefang Gao0Yubo Zhou1Qiang Zhang2Kun Zhang3Pai Peng4Liangchao Chen5Bin Xiao6College of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Forestry, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Forestry, Northwest A&F University, Yangling 712100, China; Corresponding authors.College of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, China; Corresponding authors.Five subfractions of functional polysaccharides were extracted by hydrothermal technology from Chinese tea Zhongcha 108, followed by purification using DEAE-52 column chromatography. Their structural characteristics were investigated by sugar analysis, GPC, FT-IR, AFM, methylation analysis, and 2D HSQC NMR spectrum. The results showed that the tea functional polysaccharides were mainly consisted of acidic polysaccharide rich in glucuronic acid and neutral polysaccharides composition of rhamnose, galactose, and glucose. They had a backbone of 1,4-linked α-d-Glcp and 1,2,4-linked α-l-Rhap with side chains of 1- and 1,6-linked α-d-Galp substituted at C-2 of α-l-Rhap. Furthermore, anti-cancer activities of the polysaccharides were evaluated by inhibition assays of HeLa cells proliferation. Interestingly, it was found that the acidic polysaccharide showed markedly increased effect while neutral polysaccharides F0.1-F0.3 exhibited markedly inhibitory effects on the proliferation of HeLa cells, the highest inhibition rate can reach up to 10.43% treated by F0.3 at 400 μg/ml for 48 h.http://www.sciencedirect.com/science/article/pii/S1756464617305819Tea polysaccharidesHydrothermal extractionStructureAnti-cancer activityHeLa cells |
| spellingShingle | Yuefang Gao Yubo Zhou Qiang Zhang Kun Zhang Pai Peng Liangchao Chen Bin Xiao Hydrothermal extraction, structural characterization, and inhibition HeLa cells proliferation of functional polysaccharides from Chinese tea Zhongcha 108 Journal of Functional Foods Tea polysaccharides Hydrothermal extraction Structure Anti-cancer activity HeLa cells |
| title | Hydrothermal extraction, structural characterization, and inhibition HeLa cells proliferation of functional polysaccharides from Chinese tea Zhongcha 108 |
| title_full | Hydrothermal extraction, structural characterization, and inhibition HeLa cells proliferation of functional polysaccharides from Chinese tea Zhongcha 108 |
| title_fullStr | Hydrothermal extraction, structural characterization, and inhibition HeLa cells proliferation of functional polysaccharides from Chinese tea Zhongcha 108 |
| title_full_unstemmed | Hydrothermal extraction, structural characterization, and inhibition HeLa cells proliferation of functional polysaccharides from Chinese tea Zhongcha 108 |
| title_short | Hydrothermal extraction, structural characterization, and inhibition HeLa cells proliferation of functional polysaccharides from Chinese tea Zhongcha 108 |
| title_sort | hydrothermal extraction structural characterization and inhibition hela cells proliferation of functional polysaccharides from chinese tea zhongcha 108 |
| topic | Tea polysaccharides Hydrothermal extraction Structure Anti-cancer activity HeLa cells |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617305819 |
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