Chemical analysis of atherosclerotic plaque cholesterol combined with histology of the same tissue

Chemical analysis of atherosclerotic plaque cholesterol combined with histology of the same tissue

Sensitive method for chemical analysis of free cholesterol (FC) and cholesterol esters (CE) was developed. Mouse arteries were dissected and placed in chloroform-methanol without tissue grinding. Extracts underwent hydrolysis of cholesteryl esters and derivatization of cholesterol followed by liquid...

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Bibliographic Details
Main Authors: Ming-Shang Kuo, J. Michael Kalbfleisch, Pamela Rutherford, Donetta Gifford-Moore, Xiao-di Huang, Robert Christie, Kwan Hui, Kenneth Gould, Mark Rekhter
Format: Article
Language:English
Published: Elsevier 2008-06-01
Series:Journal of Lipid Research
Subjects:
quantification
liquid chromatography
mass spectrometry
atherosclerosis
animal models
transgenic mice
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752042379X

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http://www.sciencedirect.com/science/article/pii/S002222752042379X

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