Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing

Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing

Abstract Overlap extension polymerase chain reaction (PCR) is a powerful technology for DNA assembly. Based on this technology, we synthesized DNA templates, which were transcribed into sgRNA in vitro, and further detected their efficiency of purified sgRNAs with Cas9 nuclease. The sgRNAs synthesize...

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Bibliographic Details
Main Authors: Zheng Hu, Li Wang, Zhaoying Shi, Jing Jiang, Xiangning Li, Yonglong Chen, Kai Li, Dixian Luo
Format: Article
Language:English
Published: BMC 2019-10-01
Series:Cell & Bioscience
Subjects:
Overlap extension PCR
Multiple overlapping primers
sgRNA
Transcription template
Cas9 nuclease
Online Access:http://link.springer.com/article/10.1186/s13578-019-0350-7

Internet

http://link.springer.com/article/10.1186/s13578-019-0350-7

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