Most human proteins made in both nucleus and cytoplasm turn over within minutes.

In bacteria, protein synthesis can be coupled to transcription, but in eukaryotes it is believed to occur solely in the cytoplasm. Using pulses as short as 5 s, we find that three analogues--L-azidohomoalanine, puromycin (detected after attaching fluors using 'click' chemistry or immuno-la...

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Bibliographic Details
Main Authors: Sabyasachi Baboo, Bhaskar Bhushan, Haibo Jiang, Chris R M Grovenor, Philippe Pierre, Benjamin G Davis, Peter R Cook
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4050049?pdf=render