Extension of Mitogenome Enrichment Based on Single Long-Range PCR: mtDNAs and Putative Mitochondrial-Derived Peptides of Five Rodent Hibernators

Extension of Mitogenome Enrichment Based on Single Long-Range PCR: mtDNAs and Putative Mitochondrial-Derived Peptides of Five Rodent Hibernators

Enriching mitochondrial DNA (mtDNA) for sequencing entire mitochondrial genomes (mitogenomes) can be achieved by single long-range PCR. This avoids interference from the omnipresent nuclear mtDNA sequences (NUMTs). The approach is currently restricted to the use of samples collected from humans and...

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Bibliographic Details
Main Authors: Sarah V. Emser, Helmut Schaschl, Eva Millesi, Ralf Steinborn
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-12-01
Series:Frontiers in Genetics
Subjects:
mitogenome
long-range PCR
back-to-back amplification primers
next-generation sequencing
mitochondrial-derived peptides
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2021.685806/full

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https://www.frontiersin.org/articles/10.3389/fgene.2021.685806/full

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